Time-lapse imaging of nuclear bodies

Saskia Hutten, Samuel Swift, Angus I. Lamond (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Fluorescence microscopy is a powerful technique that has become central in the study of the structure and function of biological specimens. This is due in large part to its specifi city and versatility. Although an understanding of structure—typically through high-resolution imaging of fi xed material—has proved an
important tool to understanding function, fl uorescence microscopy also offers a mechanism to interrogate cells in the living state, providing a means to explore dynamic process within the specimen over long time periods at high temporal resolution. The cell nucleus is a highly compartmented environment whose components are often highly motile and in a constant state of fl ux. The ability to monitor the dynamic behavior of nuclear bodies by live-cell imaging provides the researcher with important information regarding underlying
mechanistic processes relating to their formation and maintenance. Two techniques have proved particularly valuable to our study of cellular dynamics and molecular mobility, namely, time-lapse imaging and fl uorescence recovery after photobleaching (FRAP). Time-lapse microscopy allows for qualitative and
quantitative analysis of a wide range of events at the cellular and subcellular level. FRAP provides a mechanism to study the mobility of a population of proteins in a range of conditions within discrete areas of the biological specimen. Therefore, fl uorescence microscopy is unique in its ability to provide data at high temporal resolution and in such exquisite detail.
Original languageEnglish
Title of host publicationNuclear bodies and noncoding RNAs
Subtitle of host publicationmethods and protocols
EditorsShinichi Nakagawa, Tetsuro Hirose
Place of PublicationNew York
PublisherSpringer
Pages55-67
Number of pages13
ISBN (Electronic)9781493922536
ISBN (Print)9781493922529
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume1262
ISSN (Print)1064-3745

Keywords

  • Nuclear bodies
  • Time-lapse imaging
  • Fluorescent proteins
  • FRAP
  • Protein dynamics
  • Turnover
  • Live-cell imaging
  • Widefield microscopy

Fingerprint Dive into the research topics of 'Time-lapse imaging of nuclear bodies'. Together they form a unique fingerprint.

  • Cite this

    Hutten, S., Swift, S., & Lamond, A. I. (2015). Time-lapse imaging of nuclear bodies. In S. Nakagawa, & T. Hirose (Eds.), Nuclear bodies and noncoding RNAs: methods and protocols (pp. 55-67). (Methods in molecular biology; Vol. 1262). Springer . https://doi.org/10.1007/978-1-4939-2253-6_4