Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Judit Pampalona, Jens Januschke, Paula Sampaio, Cayetano Gonzalez

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

    Original languageEnglish
    Pages (from-to)301-315
    Number of pages15
    JournalMethods in Cell Biology
    Volume129
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Cell culture
    • Centriole
    • Centrosome
    • Drosophila
    • Microscopy
    • Neuroblast

    Fingerprint Dive into the research topics of 'Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts'. Together they form a unique fingerprint.

    Cite this