Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Judit Pampalona; Jens Januschke; Paula Sampaio; Cayetano Gonzalez

doi:10.1016/bs.mcb.2015.03.003

Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Judit Pampalona, Jens Januschke, Paula Sampaio, Cayetano Gonzalez

University of Dundee

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

Original language	English
Pages (from-to)	301-315
Number of pages	15
Journal	Methods in Cell Biology
Volume	129
DOIs	https://doi.org/10.1016/bs.mcb.2015.03.003
Publication status	Published - 2015

Keywords

Cell culture
Centriole
Centrosome
Drosophila
Microscopy
Neuroblast

ASJC Scopus subject areas

Cell Biology

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10.1016/bs.mcb.2015.03.003

Cite this

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title = "Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts",

abstract = "Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.",

keywords = "Cell culture, Centriole, Centrosome, Drosophila, Microscopy, Neuroblast",

author = "Judit Pampalona and Jens Januschke and Paula Sampaio and Cayetano Gonzalez",

year = "2015",

doi = "10.1016/bs.mcb.2015.03.003",

language = "English",

volume = "129",

pages = "301--315",

journal = "Methods in Cell Biology",

issn = "0091-679X",

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TY - JOUR

T1 - Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

AU - Pampalona, Judit

AU - Januschke, Jens

AU - Sampaio, Paula

AU - Gonzalez, Cayetano

PY - 2015

Y1 - 2015

N2 - Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

AB - Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

KW - Cell culture

KW - Centriole

KW - Centrosome

KW - Drosophila

KW - Microscopy

KW - Neuroblast

UR - http://www.scopus.com/inward/record.url?scp=84929832565&partnerID=8YFLogxK

U2 - 10.1016/bs.mcb.2015.03.003

DO - 10.1016/bs.mcb.2015.03.003

M3 - Article

C2 - 26175445

SN - 0091-679X

VL - 129

SP - 301

EP - 315

JO - Methods in Cell Biology

JF - Methods in Cell Biology

ER -

Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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Recycling Polarity - Mechanisms Controlling Stem Cell Polarity in Consecutive Divisions in the Developing Drosophila Central Nervous System (Sir Henry Dale Fellowship)

Cite this

Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Recycling Polarity - Mechanisms Controlling Stem Cell Polarity in Consecutive Divisions in the Developing Drosophila Central Nervous System (Sir Henry Dale Fellowship)

Cite this