Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor

Ahmet T. Erdogan, Gareth O.S. Williams, Andrea Usai, Nikola Krstajic, Neil Finlayson, Andrew Beavil, Rebecca Beavil, Robert K. Henderson

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)
    71 Downloads (Pure)

    Abstract

    We demonstrate a new 512x16 single photon avalanche diode (SPAD) based line sensor with per-pixel TCSPC histogramming for time-resolved, time-zoomable, FRET spectroscopy. The line sensor can operate in single photon counting (SPC) mode as well as time-correlated single photon counting (TCSPC) and per-pixel histogramming modes. TCSPC has been the preferred method for fluorescence lifetime measurements due to its collection of full decays as a histogram of arrival times. However, TCSPC is slow due to only capturing one photon per exposure and large timestamp data transfer requirements for offline histogramming. On-chip histogramming improves the data rate by allowing multiple SPAD pulses (up to one pulse per laser period) to be processed in each exposure cycle, along with secondly reducing the I/O bottleneck as only the final histogram is transferred. This can enable 50x higher acquisition rates (up to 10 billion counts per second), along with time-zoomable histogramming operation from 1.6ns to 205ns with 50ps resolution. A broad spectral range can be interrogated with the sensor (450-900nm). Overall, these sensors provide a unique combination of light sensing capabilities for use in high speed, sensitive, optical instrumentation in the time/wavelength domain. We test the sensor performance by observation of fluorescence resonance energy transfer (FRET) between FAM and TAMRA and between EGFP and RFP FRET standards.

    Original languageEnglish
    Title of host publicationBiophysics, Biology and Biophotonics III
    Subtitle of host publicationThe Crossroads
    EditorsAdam Wax, Vadim Backman
    PublisherSPIE-International Society for Optical Engineering
    Volume10504
    ISBN (Electronic)9781510614932
    DOIs
    Publication statusPublished - 20 Feb 2018
    EventBiophysics, Biology and Biophotonics III: The Crossroads 2018 - San Francisco, United States
    Duration: 28 Jan 201828 Jan 2018

    Publication series

    NameBIOPHYSICS, BIOLOGY AND BIOPHOTONICS III: THE CROSSROADS
    ISSN (Print)0277-786X

    Conference

    ConferenceBiophysics, Biology and Biophotonics III: The Crossroads 2018
    CountryUnited States
    CitySan Francisco
    Period28/01/1828/01/18

    Fingerprint

    Avalanches
    Avalanche diodes
    avalanche diodes
    Fluorescence Resonance Energy Transfer
    resonance fluorescence
    Photons
    Spectrum Analysis
    energy transfer
    Spectroscopy
    sensors
    Sensors
    photons
    spectroscopy
    counting
    histograms
    Laser pulses
    Pixels
    pixels
    Data transfer
    pulses

    Keywords

    • CMOS
    • Fluorescence lifetime
    • FRET
    • Histogram
    • Single Photon Counting
    • SPAD
    • Spectroscopy
    • TCSPC

    Cite this

    Erdogan, A. T., Williams, G. O. S., Usai, A., Krstajic, N., Finlayson, N., Beavil, A., ... Henderson, R. K. (2018). Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor. In A. Wax, & V. Backman (Eds.), Biophysics, Biology and Biophotonics III: The Crossroads (Vol. 10504). [105040M] (BIOPHYSICS, BIOLOGY AND BIOPHOTONICS III: THE CROSSROADS). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2290058
    Erdogan, Ahmet T. ; Williams, Gareth O.S. ; Usai, Andrea ; Krstajic, Nikola ; Finlayson, Neil ; Beavil, Andrew ; Beavil, Rebecca ; Henderson, Robert K. / Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor. Biophysics, Biology and Biophotonics III: The Crossroads. editor / Adam Wax ; Vadim Backman. Vol. 10504 SPIE-International Society for Optical Engineering, 2018. (BIOPHYSICS, BIOLOGY AND BIOPHOTONICS III: THE CROSSROADS).
    @inproceedings{be546622b3ae4249bc0867b447bb6e65,
    title = "Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor",
    abstract = "We demonstrate a new 512x16 single photon avalanche diode (SPAD) based line sensor with per-pixel TCSPC histogramming for time-resolved, time-zoomable, FRET spectroscopy. The line sensor can operate in single photon counting (SPC) mode as well as time-correlated single photon counting (TCSPC) and per-pixel histogramming modes. TCSPC has been the preferred method for fluorescence lifetime measurements due to its collection of full decays as a histogram of arrival times. However, TCSPC is slow due to only capturing one photon per exposure and large timestamp data transfer requirements for offline histogramming. On-chip histogramming improves the data rate by allowing multiple SPAD pulses (up to one pulse per laser period) to be processed in each exposure cycle, along with secondly reducing the I/O bottleneck as only the final histogram is transferred. This can enable 50x higher acquisition rates (up to 10 billion counts per second), along with time-zoomable histogramming operation from 1.6ns to 205ns with 50ps resolution. A broad spectral range can be interrogated with the sensor (450-900nm). Overall, these sensors provide a unique combination of light sensing capabilities for use in high speed, sensitive, optical instrumentation in the time/wavelength domain. We test the sensor performance by observation of fluorescence resonance energy transfer (FRET) between FAM and TAMRA and between EGFP and RFP FRET standards.",
    keywords = "CMOS, Fluorescence lifetime, FRET, Histogram, Single Photon Counting, SPAD, Spectroscopy, TCSPC",
    author = "Erdogan, {Ahmet T.} and Williams, {Gareth O.S.} and Andrea Usai and Nikola Krstajic and Neil Finlayson and Andrew Beavil and Rebecca Beavil and Henderson, {Robert K.}",
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    Erdogan, AT, Williams, GOS, Usai, A, Krstajic, N, Finlayson, N, Beavil, A, Beavil, R & Henderson, RK 2018, Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor. in A Wax & V Backman (eds), Biophysics, Biology and Biophotonics III: The Crossroads. vol. 10504, 105040M, BIOPHYSICS, BIOLOGY AND BIOPHOTONICS III: THE CROSSROADS, SPIE-International Society for Optical Engineering, Biophysics, Biology and Biophotonics III: The Crossroads 2018, San Francisco, United States, 28/01/18. https://doi.org/10.1117/12.2290058

    Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor. / Erdogan, Ahmet T.; Williams, Gareth O.S.; Usai, Andrea; Krstajic, Nikola; Finlayson, Neil; Beavil, Andrew; Beavil, Rebecca; Henderson, Robert K.

    Biophysics, Biology and Biophotonics III: The Crossroads. ed. / Adam Wax; Vadim Backman. Vol. 10504 SPIE-International Society for Optical Engineering, 2018. 105040M (BIOPHYSICS, BIOLOGY AND BIOPHOTONICS III: THE CROSSROADS).

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    TY - GEN

    T1 - Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor

    AU - Erdogan, Ahmet T.

    AU - Williams, Gareth O.S.

    AU - Usai, Andrea

    AU - Krstajic, Nikola

    AU - Finlayson, Neil

    AU - Beavil, Andrew

    AU - Beavil, Rebecca

    AU - Henderson, Robert K.

    PY - 2018/2/20

    Y1 - 2018/2/20

    N2 - We demonstrate a new 512x16 single photon avalanche diode (SPAD) based line sensor with per-pixel TCSPC histogramming for time-resolved, time-zoomable, FRET spectroscopy. The line sensor can operate in single photon counting (SPC) mode as well as time-correlated single photon counting (TCSPC) and per-pixel histogramming modes. TCSPC has been the preferred method for fluorescence lifetime measurements due to its collection of full decays as a histogram of arrival times. However, TCSPC is slow due to only capturing one photon per exposure and large timestamp data transfer requirements for offline histogramming. On-chip histogramming improves the data rate by allowing multiple SPAD pulses (up to one pulse per laser period) to be processed in each exposure cycle, along with secondly reducing the I/O bottleneck as only the final histogram is transferred. This can enable 50x higher acquisition rates (up to 10 billion counts per second), along with time-zoomable histogramming operation from 1.6ns to 205ns with 50ps resolution. A broad spectral range can be interrogated with the sensor (450-900nm). Overall, these sensors provide a unique combination of light sensing capabilities for use in high speed, sensitive, optical instrumentation in the time/wavelength domain. We test the sensor performance by observation of fluorescence resonance energy transfer (FRET) between FAM and TAMRA and between EGFP and RFP FRET standards.

    AB - We demonstrate a new 512x16 single photon avalanche diode (SPAD) based line sensor with per-pixel TCSPC histogramming for time-resolved, time-zoomable, FRET spectroscopy. The line sensor can operate in single photon counting (SPC) mode as well as time-correlated single photon counting (TCSPC) and per-pixel histogramming modes. TCSPC has been the preferred method for fluorescence lifetime measurements due to its collection of full decays as a histogram of arrival times. However, TCSPC is slow due to only capturing one photon per exposure and large timestamp data transfer requirements for offline histogramming. On-chip histogramming improves the data rate by allowing multiple SPAD pulses (up to one pulse per laser period) to be processed in each exposure cycle, along with secondly reducing the I/O bottleneck as only the final histogram is transferred. This can enable 50x higher acquisition rates (up to 10 billion counts per second), along with time-zoomable histogramming operation from 1.6ns to 205ns with 50ps resolution. A broad spectral range can be interrogated with the sensor (450-900nm). Overall, these sensors provide a unique combination of light sensing capabilities for use in high speed, sensitive, optical instrumentation in the time/wavelength domain. We test the sensor performance by observation of fluorescence resonance energy transfer (FRET) between FAM and TAMRA and between EGFP and RFP FRET standards.

    KW - CMOS

    KW - Fluorescence lifetime

    KW - FRET

    KW - Histogram

    KW - Single Photon Counting

    KW - SPAD

    KW - Spectroscopy

    KW - TCSPC

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    Erdogan AT, Williams GOS, Usai A, Krstajic N, Finlayson N, Beavil A et al. Time-zoomable FRET spectroscopy with a 512 x16 SPAD line sensor. In Wax A, Backman V, editors, Biophysics, Biology and Biophotonics III: The Crossroads. Vol. 10504. SPIE-International Society for Optical Engineering. 2018. 105040M. (BIOPHYSICS, BIOLOGY AND BIOPHOTONICS III: THE CROSSROADS). https://doi.org/10.1117/12.2290058