Tissue culture propagation alters plant-microbe interactions in tobacco rhizosphere

E. L. Tilston, C. Halpin, D. W. Hopkins

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    We have compared properties of roots from different lines (genotypes) of tobacco raised either in tissue culture or grown from seed. The different lines included unmodified plants and plants modified to express reduced activity of the enzyme cinnamoyl-CoA reductase, which has a pivotal role in lignin biosynthesis. The size and structure of the rhizosphere microbial community, characterized by adenosine triphosphate and phospholipid fatty acid analyses, were related to root chemistry (specifically the soluble carbohydrate concentration) and decomposition rate of the roots. The root material from unmodified plants decomposed faster following tissue culture compared with seed culture, and the faster decomposing material had significantly higher soluble carbohydrate concentrations. These observations are linked to the larger microbial biomass and greater diversity of the rhizosphere communities of tissue culture propagated plants.

    Original languageEnglish
    Pages (from-to)897-901
    Number of pages5
    JournalBiology and Fertility of Soils
    Volume44
    Issue number6
    DOIs
    Publication statusPublished - Jul 2008

    Keywords

    • genetic modification
    • in vitro propagation
    • lignin
    • Nicotiana tabacum (tobacco)
    • rhizosphere microbial community
    • soluble carbohydrates
    • EX-VITRO ACCLIMATIZATION
    • GENETIC MODIFICATIONS
    • IN-VITRO
    • LIGNIN BIOSYNTHESIS
    • SOIL
    • BIOMASS
    • ACID
    • PHOTOSYNTHESIS
    • DECOMPOSITION
    • GROWTH

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