TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway

Laura D'Ignazio, Michael Batie, Sonia Rocha (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Non-canonical NF-κB signalling plays important roles in the development and function of the immune system but it also is deregulated in a number of inflammatory diseases. Although, NF-κB and HIF crosstalk has been documented, this has only been described following canonical NF-κB stimulation, involving RelA/p50 and the HIF-1 dimer. Here, we report that the non-canonical inducer TNFSF14/LIGHT leads to HIF induction and activation in cancer cells. We demonstrate that only HIF-2α is induced at the transcriptional level following non-canonical NF-κB activation, via a mechanism that is dependent on the p52 subunit. Furthermore, we demonstrate that p52 can bind to the HIF-2α promoter in cells. These results indicate that non-canonical NF-κB can lead to HIF signalling implicating HIF-2α as one of the downstream effectors of this pathway in cells.

Original languageEnglish
Article number102
Pages (from-to)1-14
Number of pages14
JournalCells
Volume7
Issue number8
DOIs
Publication statusPublished - 8 Aug 2018

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Chemical activation
Light
Immune system
Crosstalk
Dimers
Cells
Immune System
Neoplasms

Keywords

  • HIF
  • NF-κb
  • EPAS1
  • LIGHT
  • TNFSF14
  • p52
  • NIK
  • ChIP

Cite this

D'Ignazio, Laura ; Batie, Michael ; Rocha, Sonia. / TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway. In: Cells. 2018 ; Vol. 7, No. 8. pp. 1-14.
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abstract = "Non-canonical NF-κB signalling plays important roles in the development and function of the immune system but it also is deregulated in a number of inflammatory diseases. Although, NF-κB and HIF crosstalk has been documented, this has only been described following canonical NF-κB stimulation, involving RelA/p50 and the HIF-1 dimer. Here, we report that the non-canonical inducer TNFSF14/LIGHT leads to HIF induction and activation in cancer cells. We demonstrate that only HIF-2α is induced at the transcriptional level following non-canonical NF-κB activation, via a mechanism that is dependent on the p52 subunit. Furthermore, we demonstrate that p52 can bind to the HIF-2α promoter in cells. These results indicate that non-canonical NF-κB can lead to HIF signalling implicating HIF-2α as one of the downstream effectors of this pathway in cells.",
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D'Ignazio, L, Batie, M & Rocha, S 2018, 'TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway', Cells, vol. 7, no. 8, 102, pp. 1-14. https://doi.org/10.3390/cells7080102

TNFSF14/LIGHT, a Non-Canonical NF-κB Stimulus, Induces the HIF Pathway. / D'Ignazio, Laura; Batie, Michael; Rocha, Sonia (Lead / Corresponding author).

In: Cells, Vol. 7, No. 8, 102, 08.08.2018, p. 1-14.

Research output: Contribution to journalArticle

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AU - D'Ignazio, Laura

AU - Batie, Michael

AU - Rocha, Sonia

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Y1 - 2018/8/8

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