To 5D and beyond: Quantitative fluorescence microscopy in the postgenomic era

Paul D. Andrews, Ian S. Harper, Jason R. Swedlow (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    55 Citations (Scopus)


    Digital fluorescence microscopy is now a standard technology for assaying molecular localisation in cells and tissues. The choice of laser scanning (LSM) and wide-field microscopes (WFM) largely depends on the type of sample, with LSMs performing best on thick samples and WFMs performing best on thin ones. These systems are increasingly used to collect large multidimensional datasets. We propose a unified image structure that considers space, time, and fluorescence wavelength as integral parts of the image. Moreover, the application of fluorescence imaging to large-scale screening means that large datasets are now routinely acquired. We propose that analysis of these data requires querying tools based on relational databases and describe one such system.

    Original languageEnglish
    Pages (from-to)29-36
    Number of pages8
    Issue number1
    Publication statusPublished - Jan 2002


    • Confocal microscopy
    • Deconvolution
    • Microscopy
    • Restoration
    • Three-dimensional imaging

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology


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