Tools to study SUMO conjugation in Caenorhabditis elegans

Federico Pelisch (Lead / Corresponding author), Ronald T. Hay

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

    7 Citations (Scopus)

    Abstract

    The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. In recent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in the nematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, the dynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and time within living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can be addressed using recombinant purified components of the C. elegans sumoylation machinery, including E3 ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biological role of SUMO in the context of a multicellular organism.

    Original languageEnglish
    Title of host publicationSUMO
    Subtitle of host publicationmethods and protocols
    EditorsManuel S. Rodriguez
    Place of PublicationNew York
    PublisherSpringer
    Pages233-256
    Number of pages24
    ISBN (Electronic)9781493963584
    ISBN (Print)9781493963560
    DOIs
    Publication statusPublished - 2016

    Publication series

    NameMethods in molecular biology
    PublisherSpringer
    Volume1475
    ISSN (Print)1064-3745

    Keywords

    • Journal Article

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