Tools to study SUMO conjugation in Caenorhabditis elegans

Federico Pelisch (Lead / Corresponding author), Ronald T. Hay

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

5 Citations (Scopus)

Abstract

The cell biology of sumoylation has mostly been studied using transformed cultured cells and yeast. In recent years, genetic analysis has demonstrated important roles for sumoylation in the biology of C. elegans. Here, we expand the existing set of tools making it possible to address the role of sumoylation in the nematode C. elegans using a combination of genetics, imaging, and biochemistry. Most importantly, the dynamics of SUMO conjugation and deconjugation can be followed very precisely both in space and time within living worms. Additionally, the biochemistry of SUMO conjugation and deconjugation can be addressed using recombinant purified components of the C. elegans sumoylation machinery, including E3 ligases and SUMO proteases. These tools and reagents will be useful to gain insights into the biological role of SUMO in the context of a multicellular organism.

Original languageEnglish
Title of host publicationSUMO
Subtitle of host publicationmethods and protocols
EditorsManuel S. Rodriguez
Place of PublicationNew York
PublisherSpringer
Pages233-256
Number of pages24
ISBN (Electronic)9781493963584
ISBN (Print)9781493963560
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume1475
ISSN (Print)1064-3745

Keywords

  • Journal Article

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    Pelisch, F., & Hay, R. T. (2016). Tools to study SUMO conjugation in Caenorhabditis elegans. In M. S. Rodriguez (Ed.), SUMO: methods and protocols (pp. 233-256). (Methods in molecular biology; Vol. 1475). Springer . https://doi.org/10.1007/978-1-4939-6358-4_17