Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection

Suseela Yelumalai, Marc Yeste, Celine Jones, Siti N. Amdani, Junaid Kashir, Ginny Mounce, Sarah J. Martins Da Silva, Christopher L. Barratt, Enda McVeigh, Kevin Coward (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

71 Citations (Scopus)

Abstract

Objective: To study the relationship of total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta, with fertilization rates after invitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Design: Laboratory study; controls vs. patients after IVF (n = 27) or ICSI (n = 17) treatment. Setting: Fertility center. Patient(s): A total of 44 semen samples, subjected to either IVF or ICSI treatment. Oocyte collection, ICSI or IVF, determination of sperm concentration and motility, and immunocytochemical analyses of phospholipase C zeta (PLCζ). Intervention(s): None. Main Outcome Measure(s): Percentages of sperm exhibiting PLCζ. Result(s): Significant positive correlation between ICSI fertilization rates and total levels, localization patterns, and the proportion (percentage) of sperm exhibiting PLCζ. Total levels, localization patterns, and the proportion of sperm exhibiting PLCζ are correlated with fertilization rates for ICSI, but not for IVF. Conclusion(s): Evaluating total levels, localization patterns, and proportions of PLCζ may represent a useful diagnostic tool for clinical purposes in men for whom IVF is not advised or has previously failed. This clinical study further supports the fundamental role of PLCζ in the oocyte activation process.

Original languageEnglish
Pages (from-to)561-568.e4
Number of pages12
JournalFertility and Sterility
Volume104
Issue number3
Early online date18 Jun 2015
DOIs
Publication statusPublished - Sept 2015

Keywords

  • Intracytoplasmic sperm injection
  • Invitro fertilization
  • Oocyte activation
  • Phospholipase C zeta
  • Total fertilization failure

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

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