Towards single-photon deep-tissue microscopy

Tom Vettenburg

doi:10.1117/12.2573210

Towards single-photon deep-tissue microscopy

Tom Vettenburg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

192 Downloads (Pure)

Abstract

Fluorescence light-sheet microscopy is gaining rapid adoption in developmental biology. With irradiation levels well below that of confocal and multi-photon microscopy, it enables the study of intact organs and organisms for prolonged time periods during development. Minimal sample exposure is achieved by selectively illuminating the focal plane with a second objective orthogonal to the detection axis. The light-sheet microscope's ability to study intact biological samples as and when they grow highlights the importance of imaging deeper into biological samples. Yet, deep-tissue microscopy is hampered by autofluorescence and the scattering of light. Direct observations are therefore limited to highly transparent and thin samples. Here, we show how autofluorescence can be eliminated effectively by relying on reversible photoswitching fluorescence while we propose a way forward to study and control light propagation in optically-thick tissues.

Original language	English
Title of host publication	Proceedings
Subtitle of host publication	Biomedical Imaging and Sensing Conference 2020
Editors	Toyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
Publisher	SPIE-International Society for Optical Engineering
Number of pages	3
Volume	11521
ISBN (Electronic)	9781510638495
DOIs	https://doi.org/10.1117/12.2573210
Publication status	Published - 15 Jun 2020
Event	6th Biomedical Imaging and Sensing Conference - Yokohama, Japan Duration: 20 Apr 2020 → 24 Apr 2020

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	6th Biomedical Imaging and Sensing Conference
Country/Territory	Japan
City	Yokohama
Period	20/04/20 → 24/04/20

Keywords

Auto-fluorescence
Fluorescence microscopy
Optical scattering
Reversible photoswitching

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2573210

Author Accepted ManuscriptAccepted author manuscript, 322 KB

Cite this

Vettenburg, T. (2020). Towards single-photon deep-tissue microscopy. In T. Yatagai, Y. Aizu, O. Matoba, Y. Awatsuji, & Y. Luo (Eds.), Proceedings: Biomedical Imaging and Sensing Conference 2020 (Vol. 11521). Article 1152103 (Proceedings of SPIE - The International Society for Optical Engineering). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2573210

@inproceedings{816eff35f7014bf8b384a84f7091b517,

title = "Towards single-photon deep-tissue microscopy",

abstract = "Fluorescence light-sheet microscopy is gaining rapid adoption in developmental biology. With irradiation levels well below that of confocal and multi-photon microscopy, it enables the study of intact organs and organisms for prolonged time periods during development. Minimal sample exposure is achieved by selectively illuminating the focal plane with a second objective orthogonal to the detection axis. The light-sheet microscope's ability to study intact biological samples as and when they grow highlights the importance of imaging deeper into biological samples. Yet, deep-tissue microscopy is hampered by autofluorescence and the scattering of light. Direct observations are therefore limited to highly transparent and thin samples. Here, we show how autofluorescence can be eliminated effectively by relying on reversible photoswitching fluorescence while we propose a way forward to study and control light propagation in optically-thick tissues.",

keywords = "Auto-fluorescence, Fluorescence microscopy, Optical scattering, Reversible photoswitching",

author = "Tom Vettenburg",

year = "2020",

month = jun,

day = "15",

doi = "10.1117/12.2573210",

language = "English",

volume = "11521",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE-International Society for Optical Engineering",

editor = "Toyohiko Yatagai and Yoshihisa Aizu and Osamu Matoba and Yasuhiro Awatsuji and Yuan Luo",

booktitle = "Proceedings",

note = "6th Biomedical Imaging and Sensing Conference ; Conference date: 20-04-2020 Through 24-04-2020",

}

Vettenburg, T 2020, Towards single-photon deep-tissue microscopy. in T Yatagai, Y Aizu, O Matoba, Y Awatsuji & Y Luo (eds), Proceedings: Biomedical Imaging and Sensing Conference 2020. vol. 11521, 1152103, Proceedings of SPIE - The International Society for Optical Engineering, SPIE-International Society for Optical Engineering, 6th Biomedical Imaging and Sensing Conference, Yokohama, Japan, 20/04/20. https://doi.org/10.1117/12.2573210

Towards single-photon deep-tissue microscopy. / Vettenburg, Tom.
Proceedings: Biomedical Imaging and Sensing Conference 2020. ed. / Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo. Vol. 11521 SPIE-International Society for Optical Engineering, 2020. 1152103 (Proceedings of SPIE - The International Society for Optical Engineering).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Towards single-photon deep-tissue microscopy

AU - Vettenburg, Tom

PY - 2020/6/15

Y1 - 2020/6/15

N2 - Fluorescence light-sheet microscopy is gaining rapid adoption in developmental biology. With irradiation levels well below that of confocal and multi-photon microscopy, it enables the study of intact organs and organisms for prolonged time periods during development. Minimal sample exposure is achieved by selectively illuminating the focal plane with a second objective orthogonal to the detection axis. The light-sheet microscope's ability to study intact biological samples as and when they grow highlights the importance of imaging deeper into biological samples. Yet, deep-tissue microscopy is hampered by autofluorescence and the scattering of light. Direct observations are therefore limited to highly transparent and thin samples. Here, we show how autofluorescence can be eliminated effectively by relying on reversible photoswitching fluorescence while we propose a way forward to study and control light propagation in optically-thick tissues.

AB - Fluorescence light-sheet microscopy is gaining rapid adoption in developmental biology. With irradiation levels well below that of confocal and multi-photon microscopy, it enables the study of intact organs and organisms for prolonged time periods during development. Minimal sample exposure is achieved by selectively illuminating the focal plane with a second objective orthogonal to the detection axis. The light-sheet microscope's ability to study intact biological samples as and when they grow highlights the importance of imaging deeper into biological samples. Yet, deep-tissue microscopy is hampered by autofluorescence and the scattering of light. Direct observations are therefore limited to highly transparent and thin samples. Here, we show how autofluorescence can be eliminated effectively by relying on reversible photoswitching fluorescence while we propose a way forward to study and control light propagation in optically-thick tissues.

KW - Auto-fluorescence

KW - Fluorescence microscopy

KW - Optical scattering

KW - Reversible photoswitching

UR - http://www.scopus.com/inward/record.url?scp=85087953855&partnerID=8YFLogxK

U2 - 10.1117/12.2573210

DO - 10.1117/12.2573210

M3 - Conference contribution

AN - SCOPUS:85087953855

VL - 11521

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Proceedings

A2 - Yatagai, Toyohiko

A2 - Aizu, Yoshihisa

A2 - Matoba, Osamu

A2 - Awatsuji, Yasuhiro

A2 - Luo, Yuan

PB - SPIE-International Society for Optical Engineering

T2 - 6th Biomedical Imaging and Sensing Conference

Y2 - 20 April 2020 through 24 April 2020

ER -

Towards single-photon deep-tissue microscopy

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Correlative Refractive Index Light-Sheet Microscopy

Cite this

Towards single-photon deep-tissue microscopy

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Correlative Refractive Index Light-Sheet Microscopy

Cite this