Towards single-photon deep-tissue microscopy

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Abstract

Fluorescence light-sheet microscopy is gaining rapid adoption in developmental biology. With irradiation levels well below that of confocal and multi-photon microscopy, it enables the study of intact organs and organisms for prolonged time periods during development. Minimal sample exposure is achieved by selectively illuminating the focal plane with a second objective orthogonal to the detection axis. The light-sheet microscope's ability to study intact biological samples as and when they grow highlights the importance of imaging deeper into biological samples. Yet, deep-tissue microscopy is hampered by autofluorescence and the scattering of light. Direct observations are therefore limited to highly transparent and thin samples. Here, we show how autofluorescence can be eliminated effectively by relying on reversible photoswitching fluorescence while we propose a way forward to study and control light propagation in optically-thick tissues.

Original languageEnglish
Title of host publicationProceedings
Subtitle of host publicationBiomedical Imaging and Sensing Conference 2020
EditorsToyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
PublisherSPIE-International Society for Optical Engineering
Number of pages3
Volume11521
ISBN (Electronic)9781510638495
DOIs
Publication statusPublished - 15 Jun 2020
Event6th Biomedical Imaging and Sensing Conference - Yokohama, Japan
Duration: 20 Apr 202024 Apr 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference6th Biomedical Imaging and Sensing Conference
CountryJapan
CityYokohama
Period20/04/2024/04/20

Keywords

  • Auto-fluorescence
  • Fluorescence microscopy
  • Optical scattering
  • Reversible photoswitching

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  • Cite this

    Vettenburg, T. (2020). Towards single-photon deep-tissue microscopy. In T. Yatagai, Y. Aizu, O. Matoba, Y. Awatsuji, & Y. Luo (Eds.), Proceedings: Biomedical Imaging and Sensing Conference 2020 (Vol. 11521). [1152103] (Proceedings of SPIE - The International Society for Optical Engineering). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2573210