Tracking disease resistance deployment in potato breeding by enrichment sequencing

Miles Armstrong; Jack H. Vossen; Joanne Tze-Yin Lim; Ronald C. B. Hutten; Jianfei  Xu; Shona M. Strachan; Brian Harrower; Nicolas Champouret; Eleanor M. Gilroy; Ingo Hein

doi:10.1111/pbi.12997

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Miles Armstrong, Jack H. Vossen, Joanne Tze-Yin Lim, Ronald C. B. Hutten, Jianfei Xu, Shona M. Strachan, Brian Harrower, Nicolas Champouret, Eleanor M. Gilroy, Ingo Hein (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

251 Downloads (Pure)

Abstract

Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the highconfidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

Original language	English
Pages (from-to)	540-549
Number of pages	10
Journal	Plant Biotechnology Journal
Volume	17
Issue number	2
Early online date	14 Aug 2018
DOIs	https://doi.org/10.1111/pbi.12997
Publication status	Published - Feb 2019

Keywords

Disease Resistance Genes
dRenSeq
Crops
Breeding
Tracking of NLRs
potato
tracking of NLRs
crops
breeding
disease resistance genes
Plant Breeding
Disease Resistance/genetics
Polymorphism, Genetic
Tetraploidy
Plant Proteins/genetics
Plants, Genetically Modified
Plant Diseases/immunology
Crops, Agricultural
Solanum tuberosum/genetics
Phytophthora infestans/immunology

ASJC Scopus subject areas

Agronomy and Crop Science
Biotechnology
Plant Science

Access to Document

10.1111/pbi.12997Licence: CC BY

Final Published VersionFinal published version, 1.08 MBLicence: CC BY

Studying Co Evolution in Agriculture to Inform NLR Deployment (Industrial Partnership Award - IPA)
Hein, I. (Investigator)
Biotechnology and Biological Sciences Research Council
1/12/19 → 31/08/23
Project: Research

Hein, Ingo
- Plant Sciences - Professor (Teaching and Research) of Crop Genetics
Person: Academic

Cite this

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title = "Tracking disease resistance deployment in potato breeding by enrichment sequencing",

abstract = "Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the highconfidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.",

keywords = "Disease Resistance Genes, dRenSeq, Crops, Breeding, Tracking of NLRs, potato, tracking of NLRs, crops, breeding, disease resistance genes, Plant Breeding, Disease Resistance/genetics, Polymorphism, Genetic, Tetraploidy, Plant Proteins/genetics, Plants, Genetically Modified, Plant Diseases/immunology, Crops, Agricultural, Solanum tuberosum/genetics, Phytophthora infestans/immunology",

author = "Miles Armstrong and Vossen, {Jack H.} and Lim, {Joanne Tze-Yin} and Hutten, {Ronald C. B.} and Jianfei Xu and Strachan, {Shona M.} and Brian Harrower and Nicolas Champouret and Gilroy, {Eleanor M.} and Ingo Hein",

note = "The work was funded through BBSRC awards: BB/L008025/1 and BB/K018299/1. ",

year = "2019",

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doi = "10.1111/pbi.12997",

language = "English",

volume = "17",

pages = "540--549",

journal = "Plant Biotechnology Journal",

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AU - Armstrong, Miles

AU - Vossen, Jack H.

AU - Lim, Joanne Tze-Yin

AU - Hutten, Ronald C. B.

AU - Xu, Jianfei

AU - Strachan, Shona M.

AU - Harrower, Brian

AU - Champouret, Nicolas

AU - Gilroy, Eleanor M.

AU - Hein, Ingo

N1 - The work was funded through BBSRC awards: BB/L008025/1 and BB/K018299/1.

PY - 2019/2

Y1 - 2019/2

N2 - Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the highconfidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

AB - Following the molecular characterisation of functional disease resistance genes in recent years, methods to track and verify the integrity of multiple genes in varieties are needed for crop improvement through resistance stacking. Diagnostic resistance gene enrichment sequencing (dRenSeq) enables the highconfidence identification and complete sequence validation of known functional resistance genes in crops. As demonstrated for tetraploid potato varieties, the methodology is more robust and cost-effective in monitoring resistances than whole-genome sequencing and can be used to appraise (trans)gene integrity efficiently. All currently known NB-LRRs effective against viruses, nematodes and the late blight pathogen Phytophthora infestans can be tracked with dRenSeq in potato and hitherto unknown polymorphisms have been identified. The methodology provides a means to improve the speed and efficiency of future disease resistance breeding in crops by directing parental and progeny selection towards effective combinations of resistance genes.

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KW - Breeding

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KW - tracking of NLRs

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KW - breeding

KW - disease resistance genes

KW - Plant Breeding

KW - Disease Resistance/genetics

KW - Polymorphism, Genetic

KW - Tetraploidy

KW - Plant Proteins/genetics

KW - Plants, Genetically Modified

KW - Plant Diseases/immunology

KW - Crops, Agricultural

KW - Solanum tuberosum/genetics

KW - Phytophthora infestans/immunology

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DO - 10.1111/pbi.12997

M3 - Article

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SP - 540

EP - 549

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

IS - 2

ER -

Tracking disease resistance deployment in potato breeding by enrichment sequencing

Abstract

Keywords

ASJC Scopus subject areas

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Projects

Studying Co Evolution in Agriculture to Inform NLR Deployment (Industrial Partnership Award - IPA)

Profiles

Hein, Ingo

Cite this