TRAF4 is a novel phosphoinositide-binding protein modulating tight junctions and favoring cell migration

Adrien Rousseau, Alastair G McEwen, Pierre Poussin-Courmontagne, Didier Rognan, Yves Nominé, Marie-Christine Rio, Catherine Tomasetto, Fabien Alpy (Lead / Corresponding author)

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Abstract

Tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4) is frequently overexpressed in carcinomas, suggesting a specific role in cancer. Although TRAF4 protein is predominantly found at tight junctions (TJs) in normal mammary epithelial cells (MECs), it accumulates in the cytoplasm of malignant MECs. How TRAF4 is recruited and functions at TJs is unclear. Here we show that TRAF4 possesses a novel phosphoinositide (PIP)-binding domain crucial for its recruitment to TJs. Of interest, this property is shared by the other members of the TRAF protein family. Indeed, the TRAF domain of all TRAF proteins (TRAF1 to TRAF6) is a bona fide PIP-binding domain. Molecular and structural analyses revealed that the TRAF domain of TRAF4 exists as a trimer that binds up to three lipids using basic residues exposed at its surface. Cellular studies indicated that TRAF4 acts as a negative regulator of TJ and increases cell migration. These functions are dependent from its ability to interact with PIPs. Our results suggest that TRAF4 overexpression might contribute to breast cancer progression by destabilizing TJs and favoring cell migration.

Original languageEnglish
Article numbere1001726
Number of pages21
JournalPLoS Biology
Volume11
Issue number12
DOIs
Publication statusPublished - 3 Dec 2013

Keywords

  • Animals
  • COS Cells
  • Cell Membrane/physiology
  • Cell Movement/physiology
  • Cercopithecus aethiops
  • Humans
  • Phosphatidylinositols/physiology
  • Recombinant Proteins
  • TNF Receptor-Associated Factor 4/physiology
  • Tight Junctions/physiology

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    Rousseau, A., McEwen, A. G., Poussin-Courmontagne, P., Rognan, D., Nominé, Y., Rio, M-C., Tomasetto, C., & Alpy, F. (2013). TRAF4 is a novel phosphoinositide-binding protein modulating tight junctions and favoring cell migration. PLoS Biology, 11(12), [e1001726]. https://doi.org/10.1371/journal.pbio.1001726