Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions

Peter Thorpe, C. M. Escudero‐Martinez, S. Eves-van den Akker, J. I. B. Bos (Lead / Corresponding author)

Research output: Contribution to journalArticle

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Abstract

Aphids feature complex life cycles, which in the case of many agriculturally important species involves primary and secondary host plant species. Whilst host alternation between primary and secondary host can occur in the field depending on host availability and the environment, aphid populations maintained as laboratory stocks generally are kept under conditions that allow asexual reproduction by parthenogenesis on secondary hosts. We used Myzus cerasi (black cherry aphid) to assess aphid transcriptional differences between populations collected from primary hosts in the field and those adapted to secondary hosts under controlled environment conditions. Transfer of M. cerasi collected from local cherry tress to reported secondary host species resulted in low survival rates. Moreover, aphids were unable to survive on secondary host Land cress, unless first adapted to another secondary host, cleavers. Transcriptome analyses of the different aphid populations (field collected and adapted) revealed extensive transcriptional plasticity to a change in environment, with predominantly genes involved in redox reactions differentially regulated. Most of the differentially expressed genes were duplicated and we found evidence for differential exon usage. Our data suggest that aphid adaptation to different environments may pose a major hurdle and leads to extensive gene expression changes. This article is protected by copyright. All rights reserved.

Original languageEnglish
Number of pages12
JournalInsect Molecular Biology
Early online date17 Dec 2019
DOIs
Publication statusE-pub ahead of print - 17 Dec 2019

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Aphids
Galium
Asexual Reproduction
Parthenogenesis
Population
Brassicaceae
Controlled Environment
Gene Expression Profiling
Life Cycle Stages
Genes
Oxidation-Reduction
Exons
Survival Rate
Gene Expression

Keywords

  • aphid host adaptation
  • laboratory environment
  • RNAseq
  • detoxification

Cite this

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title = "Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions",
abstract = "Aphids feature complex life cycles, which in the case of many agriculturally important species involves primary and secondary host plant species. Whilst host alternation between primary and secondary host can occur in the field depending on host availability and the environment, aphid populations maintained as laboratory stocks generally are kept under conditions that allow asexual reproduction by parthenogenesis on secondary hosts. We used Myzus cerasi (black cherry aphid) to assess aphid transcriptional differences between populations collected from primary hosts in the field and those adapted to secondary hosts under controlled environment conditions. Transfer of M. cerasi collected from local cherry tress to reported secondary host species resulted in low survival rates. Moreover, aphids were unable to survive on secondary host Land cress, unless first adapted to another secondary host, cleavers. Transcriptome analyses of the different aphid populations (field collected and adapted) revealed extensive transcriptional plasticity to a change in environment, with predominantly genes involved in redox reactions differentially regulated. Most of the differentially expressed genes were duplicated and we found evidence for differential exon usage. Our data suggest that aphid adaptation to different environments may pose a major hurdle and leads to extensive gene expression changes. This article is protected by copyright. All rights reserved.",
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author = "Peter Thorpe and Escudero‐Martinez, {C. M.} and {Eves-van den Akker}, S. and Bos, {J. I. B.}",
note = "We thank Brian Fenton for help with adapting Myzus cerasi collected from cherry trees to secondary hosts, and Melanie Febrer for advice on the RNAseq experiment and sample processing. This work was supported by the Biotechnology and Biological Sciences (BB/R011311/ to SEvdA), European Research Council (310190-APHIDHOST to JIBB), and Royal Society of Edinburgh (fellowship to JIBB). Bioinformatics and computational analyses were supported by the University of St Andrews Bioinformatics Unit, which is funded by a Wellcome Trust ISSF award (grant 105621/Z/14/Z).",
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Transcriptional changes in the aphid species Myzus cerasi under different host and environmental conditions. / Thorpe, Peter; Escudero‐Martinez, C. M.; Eves-van den Akker, S. ; Bos, J. I. B. (Lead / Corresponding author).

In: Insect Molecular Biology, 17.12.2019.

Research output: Contribution to journalArticle

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AU - Escudero‐Martinez, C. M.

AU - Eves-van den Akker, S.

AU - Bos, J. I. B.

N1 - We thank Brian Fenton for help with adapting Myzus cerasi collected from cherry trees to secondary hosts, and Melanie Febrer for advice on the RNAseq experiment and sample processing. This work was supported by the Biotechnology and Biological Sciences (BB/R011311/ to SEvdA), European Research Council (310190-APHIDHOST to JIBB), and Royal Society of Edinburgh (fellowship to JIBB). Bioinformatics and computational analyses were supported by the University of St Andrews Bioinformatics Unit, which is funded by a Wellcome Trust ISSF award (grant 105621/Z/14/Z).

PY - 2019/12/17

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N2 - Aphids feature complex life cycles, which in the case of many agriculturally important species involves primary and secondary host plant species. Whilst host alternation between primary and secondary host can occur in the field depending on host availability and the environment, aphid populations maintained as laboratory stocks generally are kept under conditions that allow asexual reproduction by parthenogenesis on secondary hosts. We used Myzus cerasi (black cherry aphid) to assess aphid transcriptional differences between populations collected from primary hosts in the field and those adapted to secondary hosts under controlled environment conditions. Transfer of M. cerasi collected from local cherry tress to reported secondary host species resulted in low survival rates. Moreover, aphids were unable to survive on secondary host Land cress, unless first adapted to another secondary host, cleavers. Transcriptome analyses of the different aphid populations (field collected and adapted) revealed extensive transcriptional plasticity to a change in environment, with predominantly genes involved in redox reactions differentially regulated. Most of the differentially expressed genes were duplicated and we found evidence for differential exon usage. Our data suggest that aphid adaptation to different environments may pose a major hurdle and leads to extensive gene expression changes. This article is protected by copyright. All rights reserved.

AB - Aphids feature complex life cycles, which in the case of many agriculturally important species involves primary and secondary host plant species. Whilst host alternation between primary and secondary host can occur in the field depending on host availability and the environment, aphid populations maintained as laboratory stocks generally are kept under conditions that allow asexual reproduction by parthenogenesis on secondary hosts. We used Myzus cerasi (black cherry aphid) to assess aphid transcriptional differences between populations collected from primary hosts in the field and those adapted to secondary hosts under controlled environment conditions. Transfer of M. cerasi collected from local cherry tress to reported secondary host species resulted in low survival rates. Moreover, aphids were unable to survive on secondary host Land cress, unless first adapted to another secondary host, cleavers. Transcriptome analyses of the different aphid populations (field collected and adapted) revealed extensive transcriptional plasticity to a change in environment, with predominantly genes involved in redox reactions differentially regulated. Most of the differentially expressed genes were duplicated and we found evidence for differential exon usage. Our data suggest that aphid adaptation to different environments may pose a major hurdle and leads to extensive gene expression changes. This article is protected by copyright. All rights reserved.

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