Transcriptional networks controlling the cell cycle

Martin Bonke, Mikko Turunen, Maria Sokolova, Anna Vähärautio, Teemu Kivioja, Minna Taipale, Mikael Bjorklund, Jussi Taipale

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    In this work, we map the transcriptional targets of 107 previously identified Drosophila genes whose loss caused the strongest cell-cycle phenotypes in a genome-wide RNA interference screen and mine the resulting data computationally. Besides confirming existing knowledge, the analysis revealed several regulatory systems, among which were two highly-specific and interconnected feedback circuits, one between the ribosome and the proteasome that controls overall protein homeostasis, and the other between the ribosome and Myc/Max that regulates the protein synthesis capacity of cells. We also identified a set of genes that alter the timing of mitosis without affecting gene expression, indicating that the cyclic transcriptional program that produces the components required for cell division can be partially uncoupled from the cell division process itself. These genes all have a function in a pathway that regulates the phosphorylation state of Cdk1. We provide evidence showing that this pathway is involved in regulation of cell size, indicating that a Cdk1-regulated cell size checkpoint exists in metazoans.
    Original languageEnglish
    Pages (from-to)75-90
    Number of pages16
    JournalG3 : Genes, Genomes, Genetics
    Volume3
    Issue number1
    DOIs
    Publication statusPublished - 2013

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  • Cite this

    Bonke, M., Turunen, M., Sokolova, M., Vähärautio, A., Kivioja, T., Taipale, M., Bjorklund, M., & Taipale, J. (2013). Transcriptional networks controlling the cell cycle. G3 : Genes, Genomes, Genetics, 3(1), 75-90. https://doi.org/10.1534/g3.112.004283