Transcriptional profiling of the LPS induced NF-kappaB response in macrophages

Omar Sharif; Viacheslav N. Bolshakov; Stephanie Raines; Peter Newham; Neil D. Perkins

doi:10.1186/1471-2172-8-1

Transcriptional profiling of the LPS induced NF-kappaB response in macrophages

Omar Sharif, Viacheslav N. Bolshakov, Stephanie Raines, Peter Newham, Neil D. Perkins

Research output: Contribution to journal › Article › peer-review

366 Citations (Scopus)

Abstract

Exposure of macrophages to bacterial products such as lipopolysaccharide (LPS) results in activation of the NF-kappaB transcription factor, which orchestrates a gene expression programme that underpins the macrophage-dependent immune response. These changes include the induction or repression of a wide range of genes that regulate inflammation, cell proliferation, migration and cell survival. This process is tightly regulated and loss of control is associated with conditions such as septic shock, inflammatory diseases and cancer. To study this response, it is important to have in vitro model systems that reflect the behaviour of cells in vivo. In addition, it is necessary to understand the natural differences that can occur between individuals. In this report, we have investigated and compared the LPS response in macrophage derived cell lines and peripheral blood mononuclear cell (PBMC) derived macrophages.

Original language	English
Pages (from-to)	1
Journal	BMC Immunology
Volume	8
DOIs	https://doi.org/10.1186/1471-2172-8-1
Publication status	Published - 2007

Keywords

Cell Line
DNA
Gene Expression Profiling
Humans
Lipopolysaccharides
Macrophages
NF-kappa B
Oligonucleotide Array Sequence Analysis
Protein Binding
RNA, Messenger
Transcription, Genetic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/1471-2172-8-1

Cite this

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title = "Transcriptional profiling of the LPS induced NF-kappaB response in macrophages",

abstract = "Exposure of macrophages to bacterial products such as lipopolysaccharide (LPS) results in activation of the NF-kappaB transcription factor, which orchestrates a gene expression programme that underpins the macrophage-dependent immune response. These changes include the induction or repression of a wide range of genes that regulate inflammation, cell proliferation, migration and cell survival. This process is tightly regulated and loss of control is associated with conditions such as septic shock, inflammatory diseases and cancer. To study this response, it is important to have in vitro model systems that reflect the behaviour of cells in vivo. In addition, it is necessary to understand the natural differences that can occur between individuals. In this report, we have investigated and compared the LPS response in macrophage derived cell lines and peripheral blood mononuclear cell (PBMC) derived macrophages.",

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T1 - Transcriptional profiling of the LPS induced NF-kappaB response in macrophages

AU - Sharif, Omar

AU - Bolshakov, Viacheslav N.

AU - Raines, Stephanie

AU - Newham, Peter

AU - Perkins, Neil D.

PY - 2007

Y1 - 2007

N2 - Exposure of macrophages to bacterial products such as lipopolysaccharide (LPS) results in activation of the NF-kappaB transcription factor, which orchestrates a gene expression programme that underpins the macrophage-dependent immune response. These changes include the induction or repression of a wide range of genes that regulate inflammation, cell proliferation, migration and cell survival. This process is tightly regulated and loss of control is associated with conditions such as septic shock, inflammatory diseases and cancer. To study this response, it is important to have in vitro model systems that reflect the behaviour of cells in vivo. In addition, it is necessary to understand the natural differences that can occur between individuals. In this report, we have investigated and compared the LPS response in macrophage derived cell lines and peripheral blood mononuclear cell (PBMC) derived macrophages.

AB - Exposure of macrophages to bacterial products such as lipopolysaccharide (LPS) results in activation of the NF-kappaB transcription factor, which orchestrates a gene expression programme that underpins the macrophage-dependent immune response. These changes include the induction or repression of a wide range of genes that regulate inflammation, cell proliferation, migration and cell survival. This process is tightly regulated and loss of control is associated with conditions such as septic shock, inflammatory diseases and cancer. To study this response, it is important to have in vitro model systems that reflect the behaviour of cells in vivo. In addition, it is necessary to understand the natural differences that can occur between individuals. In this report, we have investigated and compared the LPS response in macrophage derived cell lines and peripheral blood mononuclear cell (PBMC) derived macrophages.

KW - Cell Line

KW - DNA

KW - Gene Expression Profiling

KW - Humans

KW - Lipopolysaccharides

KW - Macrophages

KW - NF-kappa B

KW - Oligonucleotide Array Sequence Analysis

KW - Protein Binding

KW - RNA, Messenger

KW - Transcription, Genetic

U2 - 10.1186/1471-2172-8-1

DO - 10.1186/1471-2172-8-1

M3 - Article

C2 - 17222336

SN - 1471-2172

VL - 8

SP - 1

JO - BMC Immunology

JF - BMC Immunology

ER -

Transcriptional profiling of the LPS induced NF-kappaB response in macrophages

Abstract

Keywords

UN SDGs

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