Transcriptional profiling of the LPS induced NF-kappaB response in macrophages

Omar Sharif, Viacheslav N. Bolshakov, Stephanie Raines, Peter Newham, Neil D. Perkins

    Research output: Contribution to journalArticlepeer-review

    339 Citations (Scopus)


    Exposure of macrophages to bacterial products such as lipopolysaccharide (LPS) results in activation of the NF-kappaB transcription factor, which orchestrates a gene expression programme that underpins the macrophage-dependent immune response. These changes include the induction or repression of a wide range of genes that regulate inflammation, cell proliferation, migration and cell survival. This process is tightly regulated and loss of control is associated with conditions such as septic shock, inflammatory diseases and cancer. To study this response, it is important to have in vitro model systems that reflect the behaviour of cells in vivo. In addition, it is necessary to understand the natural differences that can occur between individuals. In this report, we have investigated and compared the LPS response in macrophage derived cell lines and peripheral blood mononuclear cell (PBMC) derived macrophages.
    Original languageEnglish
    Pages (from-to)1
    JournalBMC Immunology
    Publication statusPublished - 2007


    • Cell Line
    • DNA
    • Gene Expression Profiling
    • Humans
    • Lipopolysaccharides
    • Macrophages
    • NF-kappa B
    • Oligonucleotide Array Sequence Analysis
    • Protein Binding
    • RNA, Messenger
    • Transcription, Genetic


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