Transcriptional pulsing of a developmental gene

Jonathan R. Chubb, Tatjana Trcek, Shailesh M. Shenoy, Robert H. Singer

    Research output: Contribution to journalArticle

    446 Citations (Scopus)

    Abstract

    It has not been possible to view the transcriptional activity of a single gene within a living eukaryotic cell. It is therefore unclear how long and how frequently a gene is actively transcribed, how this is modulated during differentiation, and how transcriptional events are dynamically coordinated in cell populations. By means of an in vivo RNA detection technique [1], [2] and [3], we have directly visualized transcription of an endogenous developmental gene. We found discrete “pulses” of gene activity that turn on and off at irregular intervals. Surprisingly, the length and height of these pulses were consistent throughout development. However, there was strong developmental variation in the proportion of cells recruited to the expressing pool. Cells were more likely to reexpress than to initiate new expression, indicating that we directly observe a transcriptional memory. In addition, we used a clustering algorithm to reveal synchronous transcription initiation in neighboring cells. This study represents the first direct visualization of transcriptional pulsing in eukaryotes. Discontinuity of transcription may allow greater flexibility in the gene-expression decisions of a cell.
    Original languageEnglish
    Pages (from-to)1018-1025
    Number of pages8
    JournalCurrent Biology
    Volume16
    Issue number10
    DOIs
    Publication statusPublished - 2006

    Keywords

    • Gene expression
    • DNA
    • RNA
    • Molecular genetics

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    Chubb, J. R., Trcek, T., Shenoy, S. M., & Singer, R. H. (2006). Transcriptional pulsing of a developmental gene. Current Biology, 16(10), 1018-1025. https://doi.org/10.1016/j.cub.2006.03.092