Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation

Beatriz Nunez-Corcuera, J.L. Birch, Y. Yamada, J.G. Williams

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of this site causes ectopic expression in the prestalk region and ChIP analysis shows that DIF-1 induces binding of DimB to the pspA promoter. DIF-1 represses pspA gene expression in a suspension cell assay but this repression is abrogated in a dimB null strain. These results suggest a coupled control mechanism, whereby the same DIF-DimB signaling pathway that directly activates ecmB gene expression directly represses pspA gene expression.
Original languageEnglish
JournalPLoS ONE
Volume7
Issue number1
DOIs
Publication statusPublished - 9 Jan 2012

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