Transcriptionally correlated subcellular dynamics of MBNL1 during lens development and their implication for the molecular pathology of myotonic dystrophy type 1

Stewart M. Coleman, Alan R. Prescott, Judith E. Sleeman (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    DM1 (myotonic dystrophy type 1) is caused by elongation of a CTG repeat in the DMPK (dystrophia myotonica-protein kinase) gene. mRNA transcripts containing these CUGexp (CUG expansion) repeats form accumulations, or foci, in the nucleus of the cell. The pathogenesis of DM1 is proposed to result from inappropriate patterns of alternative splicing caused by sequestration of the developmentally regulated alternative splicing factor MBNL1 (muscleblind-like 1) by these foci. Since eye lens cataract is a common feature of DM1 we have examined the distribution and dynamics of MBNL1 in lens epithelial cell lines derived from patients with DM1. The results of the present study demonstrate that only a small proportion of nuclear MBNL1 accumulates in CUGexp pre-mRNA foci. MBNL1 is, however, highly mobile and changes localization in response to altered transcription and splicing activity. Moreover, immunolocalization studies in lens sections suggest that a change in MBNL1 distribution is important during lens growth and differentiation. Although these data suggest that the loss of MBNL1 function due to accumulation in foci is an unlikely explanation for DM1 symptoms in the lens, they do demonstrate a strong relationship between the subcellular MBNL1 localization and pathways of cellular differentiation, providing an insight into the sensitivity of the lens to changes in MBNL1 distribution.
    Original languageEnglish
    Pages (from-to)267-280
    Number of pages14
    JournalBiochemical Journal
    Volume458
    Issue number2
    DOIs
    Publication statusPublished - 1 Mar 2014

    Fingerprint

    Myotonic Dystrophy
    Molecular Pathology
    Pathology
    Lenses
    Alternative Splicing
    Crystalline Lens
    RNA Precursors
    Cell Nucleus
    Cataract
    Protein Kinases
    Transcription
    Epithelial Cells
    Elongation
    Cell Line
    Genes
    Messenger RNA
    Growth

    Keywords

    • Alternative Splicing
    • Animals
    • Cells, Cultured
    • Epithelial Cells
    • Humans
    • Lens, Crystalline
    • Molecular Dynamics Simulation
    • Myotonic Dystrophy
    • RNA-Binding Proteins
    • Subcellular Fractions
    • Swine
    • Transcription, Genetic

    Cite this

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    title = "Transcriptionally correlated subcellular dynamics of MBNL1 during lens development and their implication for the molecular pathology of myotonic dystrophy type 1",
    abstract = "DM1 (myotonic dystrophy type 1) is caused by elongation of a CTG repeat in the DMPK (dystrophia myotonica-protein kinase) gene. mRNA transcripts containing these CUGexp (CUG expansion) repeats form accumulations, or foci, in the nucleus of the cell. The pathogenesis of DM1 is proposed to result from inappropriate patterns of alternative splicing caused by sequestration of the developmentally regulated alternative splicing factor MBNL1 (muscleblind-like 1) by these foci. Since eye lens cataract is a common feature of DM1 we have examined the distribution and dynamics of MBNL1 in lens epithelial cell lines derived from patients with DM1. The results of the present study demonstrate that only a small proportion of nuclear MBNL1 accumulates in CUGexp pre-mRNA foci. MBNL1 is, however, highly mobile and changes localization in response to altered transcription and splicing activity. Moreover, immunolocalization studies in lens sections suggest that a change in MBNL1 distribution is important during lens growth and differentiation. Although these data suggest that the loss of MBNL1 function due to accumulation in foci is an unlikely explanation for DM1 symptoms in the lens, they do demonstrate a strong relationship between the subcellular MBNL1 localization and pathways of cellular differentiation, providing an insight into the sensitivity of the lens to changes in MBNL1 distribution.",
    keywords = "Alternative Splicing, Animals, Cells, Cultured, Epithelial Cells, Humans, Lens, Crystalline, Molecular Dynamics Simulation, Myotonic Dystrophy, RNA-Binding Proteins, Subcellular Fractions, Swine, Transcription, Genetic",
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    Transcriptionally correlated subcellular dynamics of MBNL1 during lens development and their implication for the molecular pathology of myotonic dystrophy type 1. / Coleman, Stewart M.; Prescott, Alan R.; Sleeman, Judith E. (Lead / Corresponding author).

    In: Biochemical Journal, Vol. 458, No. 2, 01.03.2014, p. 267-280.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Prescott, Alan R.

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    AB - DM1 (myotonic dystrophy type 1) is caused by elongation of a CTG repeat in the DMPK (dystrophia myotonica-protein kinase) gene. mRNA transcripts containing these CUGexp (CUG expansion) repeats form accumulations, or foci, in the nucleus of the cell. The pathogenesis of DM1 is proposed to result from inappropriate patterns of alternative splicing caused by sequestration of the developmentally regulated alternative splicing factor MBNL1 (muscleblind-like 1) by these foci. Since eye lens cataract is a common feature of DM1 we have examined the distribution and dynamics of MBNL1 in lens epithelial cell lines derived from patients with DM1. The results of the present study demonstrate that only a small proportion of nuclear MBNL1 accumulates in CUGexp pre-mRNA foci. MBNL1 is, however, highly mobile and changes localization in response to altered transcription and splicing activity. Moreover, immunolocalization studies in lens sections suggest that a change in MBNL1 distribution is important during lens growth and differentiation. Although these data suggest that the loss of MBNL1 function due to accumulation in foci is an unlikely explanation for DM1 symptoms in the lens, they do demonstrate a strong relationship between the subcellular MBNL1 localization and pathways of cellular differentiation, providing an insight into the sensitivity of the lens to changes in MBNL1 distribution.

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    KW - Cells, Cultured

    KW - Epithelial Cells

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