Transcriptome sequencing of an ecologically important graminivorous sawfly: a resource for marker development

N. Cook, N. Aziz, P. E. Hedley, J. Morris, L. Milne, A. J. Karley, S. F. Hubbard, J. R. Russell

    Research output: Contribution to journalReview article

    6 Citations (Scopus)

    Abstract

    Sawfly larvae (Hymenoptera: Symphyta) are an important, highly nutritious, invertebrate food source for farmland birds. Reduced numbers of farmland invertebrates are a possible factor contributing to the observed declines in many farmland bird populations associated with post-1950s intensification of agriculture. To date, studies on sawfly populations have been census-based and, therefore, the genetic factors underlying their declines are unknown. We have produced the first genetic resource for any sawfly species in the form of a de novo transcriptome assembly comprising 18,539 contiguous sequences (contigs) and 260 singletons. The assembly was sequenced using 454 pyrosequencing technology and produced 1,284 microsatellite markers for the common farmland sawfly Dolerus aeneus, which may also be useful in other closely-related species. These markers will facilitate monitoring of changes in genetic diversity and gene flow in sawfly populations therefore helping to predict extinction risk.

    Original languageEnglish
    Pages (from-to)789-795
    Number of pages7
    JournalConservation Genetics Resources
    Volume3
    Issue number4
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • Sawfly
    • 454 Pyrosequencing
    • Microsatellite
    • Hymenoptera
    • Next-generation sequencing
    • FARMLAND BIRDS
    • ABUNDANCE
    • BRITAIN
    • POPULATIONS
    • PARTRIDGE
    • DECLINES
    • EUROPE
    • INSECT
    • PLANTS
    • FOOD

    Cite this

    Cook, N., Aziz, N., Hedley, P. E., Morris, J., Milne, L., Karley, A. J., Hubbard, S. F., & Russell, J. R. (2011). Transcriptome sequencing of an ecologically important graminivorous sawfly: a resource for marker development. Conservation Genetics Resources, 3(4), 789-795. https://doi.org/10.1007/s12686-011-9459-7