Transcriptome sequencing of an ecologically important graminivorous sawfly

a resource for marker development

N. Cook, N. Aziz, P. E. Hedley, J. Morris, L. Milne, A. J. Karley, S. F. Hubbard, J. R. Russell

    Research output: Contribution to journalReview article

    5 Citations (Scopus)

    Abstract

    Sawfly larvae (Hymenoptera: Symphyta) are an important, highly nutritious, invertebrate food source for farmland birds. Reduced numbers of farmland invertebrates are a possible factor contributing to the observed declines in many farmland bird populations associated with post-1950s intensification of agriculture. To date, studies on sawfly populations have been census-based and, therefore, the genetic factors underlying their declines are unknown. We have produced the first genetic resource for any sawfly species in the form of a de novo transcriptome assembly comprising 18,539 contiguous sequences (contigs) and 260 singletons. The assembly was sequenced using 454 pyrosequencing technology and produced 1,284 microsatellite markers for the common farmland sawfly Dolerus aeneus, which may also be useful in other closely-related species. These markers will facilitate monitoring of changes in genetic diversity and gene flow in sawfly populations therefore helping to predict extinction risk.

    Original languageEnglish
    Pages (from-to)789-795
    Number of pages7
    JournalConservation Genetics Resources
    Volume3
    Issue number4
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • Sawfly
    • 454 Pyrosequencing
    • Microsatellite
    • Hymenoptera
    • Next-generation sequencing
    • FARMLAND BIRDS
    • ABUNDANCE
    • BRITAIN
    • POPULATIONS
    • PARTRIDGE
    • DECLINES
    • EUROPE
    • INSECT
    • PLANTS
    • FOOD

    Cite this

    Cook, N. ; Aziz, N. ; Hedley, P. E. ; Morris, J. ; Milne, L. ; Karley, A. J. ; Hubbard, S. F. ; Russell, J. R. / Transcriptome sequencing of an ecologically important graminivorous sawfly : a resource for marker development. In: Conservation Genetics Resources. 2011 ; Vol. 3, No. 4. pp. 789-795.
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    title = "Transcriptome sequencing of an ecologically important graminivorous sawfly: a resource for marker development",
    abstract = "Sawfly larvae (Hymenoptera: Symphyta) are an important, highly nutritious, invertebrate food source for farmland birds. Reduced numbers of farmland invertebrates are a possible factor contributing to the observed declines in many farmland bird populations associated with post-1950s intensification of agriculture. To date, studies on sawfly populations have been census-based and, therefore, the genetic factors underlying their declines are unknown. We have produced the first genetic resource for any sawfly species in the form of a de novo transcriptome assembly comprising 18,539 contiguous sequences (contigs) and 260 singletons. The assembly was sequenced using 454 pyrosequencing technology and produced 1,284 microsatellite markers for the common farmland sawfly Dolerus aeneus, which may also be useful in other closely-related species. These markers will facilitate monitoring of changes in genetic diversity and gene flow in sawfly populations therefore helping to predict extinction risk.",
    keywords = "Sawfly, 454 Pyrosequencing, Microsatellite, Hymenoptera, Next-generation sequencing, FARMLAND BIRDS, ABUNDANCE, BRITAIN, POPULATIONS, PARTRIDGE, DECLINES, EUROPE, INSECT, PLANTS, FOOD",
    author = "N. Cook and N. Aziz and Hedley, {P. E.} and J. Morris and L. Milne and Karley, {A. J.} and Hubbard, {S. F.} and Russell, {J. R.}",
    year = "2011",
    month = "10",
    doi = "10.1007/s12686-011-9459-7",
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    Cook, N, Aziz, N, Hedley, PE, Morris, J, Milne, L, Karley, AJ, Hubbard, SF & Russell, JR 2011, 'Transcriptome sequencing of an ecologically important graminivorous sawfly: a resource for marker development', Conservation Genetics Resources, vol. 3, no. 4, pp. 789-795. https://doi.org/10.1007/s12686-011-9459-7

    Transcriptome sequencing of an ecologically important graminivorous sawfly : a resource for marker development. / Cook, N.; Aziz, N.; Hedley, P. E.; Morris, J.; Milne, L.; Karley, A. J.; Hubbard, S. F. ; Russell, J. R.

    In: Conservation Genetics Resources, Vol. 3, No. 4, 10.2011, p. 789-795.

    Research output: Contribution to journalReview article

    TY - JOUR

    T1 - Transcriptome sequencing of an ecologically important graminivorous sawfly

    T2 - a resource for marker development

    AU - Cook, N.

    AU - Aziz, N.

    AU - Hedley, P. E.

    AU - Morris, J.

    AU - Milne, L.

    AU - Karley, A. J.

    AU - Hubbard, S. F.

    AU - Russell, J. R.

    PY - 2011/10

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    N2 - Sawfly larvae (Hymenoptera: Symphyta) are an important, highly nutritious, invertebrate food source for farmland birds. Reduced numbers of farmland invertebrates are a possible factor contributing to the observed declines in many farmland bird populations associated with post-1950s intensification of agriculture. To date, studies on sawfly populations have been census-based and, therefore, the genetic factors underlying their declines are unknown. We have produced the first genetic resource for any sawfly species in the form of a de novo transcriptome assembly comprising 18,539 contiguous sequences (contigs) and 260 singletons. The assembly was sequenced using 454 pyrosequencing technology and produced 1,284 microsatellite markers for the common farmland sawfly Dolerus aeneus, which may also be useful in other closely-related species. These markers will facilitate monitoring of changes in genetic diversity and gene flow in sawfly populations therefore helping to predict extinction risk.

    AB - Sawfly larvae (Hymenoptera: Symphyta) are an important, highly nutritious, invertebrate food source for farmland birds. Reduced numbers of farmland invertebrates are a possible factor contributing to the observed declines in many farmland bird populations associated with post-1950s intensification of agriculture. To date, studies on sawfly populations have been census-based and, therefore, the genetic factors underlying their declines are unknown. We have produced the first genetic resource for any sawfly species in the form of a de novo transcriptome assembly comprising 18,539 contiguous sequences (contigs) and 260 singletons. The assembly was sequenced using 454 pyrosequencing technology and produced 1,284 microsatellite markers for the common farmland sawfly Dolerus aeneus, which may also be useful in other closely-related species. These markers will facilitate monitoring of changes in genetic diversity and gene flow in sawfly populations therefore helping to predict extinction risk.

    KW - Sawfly

    KW - 454 Pyrosequencing

    KW - Microsatellite

    KW - Hymenoptera

    KW - Next-generation sequencing

    KW - FARMLAND BIRDS

    KW - ABUNDANCE

    KW - BRITAIN

    KW - POPULATIONS

    KW - PARTRIDGE

    KW - DECLINES

    KW - EUROPE

    KW - INSECT

    KW - PLANTS

    KW - FOOD

    U2 - 10.1007/s12686-011-9459-7

    DO - 10.1007/s12686-011-9459-7

    M3 - Review article

    VL - 3

    SP - 789

    EP - 795

    JO - Conservation Genetics Resources

    JF - Conservation Genetics Resources

    SN - 1877-7252

    IS - 4

    ER -