Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis

Kate J. Treharne; O. Giles Best; Anil Mehta

doi:10.1016/j.febslet.2009.07.026

Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis

Kate J. Treharne, O. Giles Best, Anil Mehta

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Tissue transglutaminase (tgase2) is a multifunctional enzyme that crosslinks proteins but also acts as a G-protein, differential functions regulated by calcium and GTP. In the epithelial cell membrane, we show that manipulation of tgase2 function by monodansylcadaverine or retinoic acid ( RA) alters the activity of a membrane-bound protein kinase, nucleoside diphosphate kinase (NDPK, nm23-H1/H2) that is known to control G-protein function. We find that NDPK function is abnormally low in cystic fibrosis but can be restored by RA treatment in vitro. Our data suggest that tgase2 is overexpressed in cystic fibrosis and affects NDPK function.

Structured summary: MINT-7219905, MINT-7219896: tgase2 (uniprotkb: P21980) physically interacts (MI: 0914) with NDPK ( uniprotkb: P15531) by anti bait coimmunoprecipitation ( MI: 0006) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Original language	English
Pages (from-to)	2789-2792
Number of pages	4
Journal	FEBS Letters
Volume	583
Issue number	17
DOIs	https://doi.org/10.1016/j.febslet.2009.07.026
Publication status	Published - 3 Sept 2009

Keywords

Fibrosing colonopathy
phosphohistidine
Cross-linking
Cystic fibrosis transmembrane regulator
HETEROTRIMERIC G-PROTEINS
RETINOIC ACID INDUCTION
TISSUE TRANSGLUTAMINASE
AIRWAY EPITHELIUM
HISTIDINE PHOSPHORYLATION
BINDING PROTEIN
SWEAT-DUCT
CELLS
ACTIVATION
CFTR

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10.1016/j.febslet.2009.07.026

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@article{e58a2976a4674d12b29532af82bab213,

title = "Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis",

abstract = "Tissue transglutaminase (tgase2) is a multifunctional enzyme that crosslinks proteins but also acts as a G-protein, differential functions regulated by calcium and GTP. In the epithelial cell membrane, we show that manipulation of tgase2 function by monodansylcadaverine or retinoic acid ( RA) alters the activity of a membrane-bound protein kinase, nucleoside diphosphate kinase (NDPK, nm23-H1/H2) that is known to control G-protein function. We find that NDPK function is abnormally low in cystic fibrosis but can be restored by RA treatment in vitro. Our data suggest that tgase2 is overexpressed in cystic fibrosis and affects NDPK function.Structured summary: MINT-7219905, MINT-7219896: tgase2 (uniprotkb: P21980) physically interacts (MI: 0914) with NDPK ( uniprotkb: P15531) by anti bait coimmunoprecipitation ( MI: 0006) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.",

keywords = "Fibrosing colonopathy, phosphohistidine, Cross-linking, Cystic fibrosis transmembrane regulator, HETEROTRIMERIC G-PROTEINS, RETINOIC ACID INDUCTION, TISSUE TRANSGLUTAMINASE, AIRWAY EPITHELIUM, HISTIDINE PHOSPHORYLATION, BINDING PROTEIN, SWEAT-DUCT, CELLS, ACTIVATION, CFTR",

author = "Treharne, {Kate J.} and Best, {O. Giles} and Anil Mehta",

year = "2009",

month = sep,

day = "3",

doi = "10.1016/j.febslet.2009.07.026",

language = "English",

volume = "583",

pages = "2789--2792",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "17",

}

TY - JOUR

T1 - Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis

AU - Treharne, Kate J.

AU - Best, O. Giles

AU - Mehta, Anil

PY - 2009/9/3

Y1 - 2009/9/3

N2 - Tissue transglutaminase (tgase2) is a multifunctional enzyme that crosslinks proteins but also acts as a G-protein, differential functions regulated by calcium and GTP. In the epithelial cell membrane, we show that manipulation of tgase2 function by monodansylcadaverine or retinoic acid ( RA) alters the activity of a membrane-bound protein kinase, nucleoside diphosphate kinase (NDPK, nm23-H1/H2) that is known to control G-protein function. We find that NDPK function is abnormally low in cystic fibrosis but can be restored by RA treatment in vitro. Our data suggest that tgase2 is overexpressed in cystic fibrosis and affects NDPK function.Structured summary: MINT-7219905, MINT-7219896: tgase2 (uniprotkb: P21980) physically interacts (MI: 0914) with NDPK ( uniprotkb: P15531) by anti bait coimmunoprecipitation ( MI: 0006) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

AB - Tissue transglutaminase (tgase2) is a multifunctional enzyme that crosslinks proteins but also acts as a G-protein, differential functions regulated by calcium and GTP. In the epithelial cell membrane, we show that manipulation of tgase2 function by monodansylcadaverine or retinoic acid ( RA) alters the activity of a membrane-bound protein kinase, nucleoside diphosphate kinase (NDPK, nm23-H1/H2) that is known to control G-protein function. We find that NDPK function is abnormally low in cystic fibrosis but can be restored by RA treatment in vitro. Our data suggest that tgase2 is overexpressed in cystic fibrosis and affects NDPK function.Structured summary: MINT-7219905, MINT-7219896: tgase2 (uniprotkb: P21980) physically interacts (MI: 0914) with NDPK ( uniprotkb: P15531) by anti bait coimmunoprecipitation ( MI: 0006) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

KW - Fibrosing colonopathy

KW - phosphohistidine

KW - Cross-linking

KW - Cystic fibrosis transmembrane regulator

KW - HETEROTRIMERIC G-PROTEINS

KW - RETINOIC ACID INDUCTION

KW - TISSUE TRANSGLUTAMINASE

KW - AIRWAY EPITHELIUM

KW - HISTIDINE PHOSPHORYLATION

KW - BINDING PROTEIN

KW - SWEAT-DUCT

KW - CELLS

KW - ACTIVATION

KW - CFTR

U2 - 10.1016/j.febslet.2009.07.026

DO - 10.1016/j.febslet.2009.07.026

M3 - Article

C2 - 19619546

SN - 0014-5793

VL - 583

SP - 2789

EP - 2792

JO - FEBS Letters

JF - FEBS Letters

IS - 17

ER -

Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis

Abstract

Keywords

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