Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8+ T lymphocytes

Thomas C. J. Tan, Van Kelly, Xiaoyan Zou, David Wright, Tony Ly, Rose Zamoyska (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)
    42 Downloads (Pure)

    Abstract

    Control of mRNA translation adjusts protein production rapidly and facilitates local cellular responses to environmental conditions. Traditionally initiation of translation is considered to be a major translational control point, however, control of peptide elongation is also important. Here we show that the function of the elongation factor, eIF5a, is regulated dynamically in naïve CD8+ T cells upon activation by post-translational modification, whereupon it facilitates translation of specific subsets of proteins. eIF5a is essential for long-term survival of effector CD8+ T cells and sequencing of nascent polypeptides indicates that the production of proteins which regulate proliferation and key effector functions, particularly the production of IFNγ and less acutely TNF production and cytotoxicity, is dependent on the presence of functional eIF5a. Control of translation in multiple immune cell lineages is required to co-ordinate immune responses and these data illustrate that translational elongation contributes to post-transcriptional regulons important for the control of inflammation.

    Original languageEnglish
    Article number7796
    Number of pages16
    JournalNature Communications
    Volume13
    DOIs
    Publication statusPublished - 17 Dec 2022

    Keywords

    • Cell biology
    • Cytotoxic T cells
    • Interferons
    • Lymphocyte activation
    • RNA

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