Translation Microscopy (TRAM) for super-resolution imaging

Zhen Qiu, Rhodri S. Wilson, Yuewei Liu, Alison R. Dun, Rebecca S. Saleeb, Dongsheng Liu, Colin Rickman, Margaret Frame, Rory R. Duncan, Weiping Lu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
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Abstract

Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ‘ground-truth’ DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.
Original languageEnglish
Article number19993
Number of pages11
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Jan 2016

Keywords

  • Computational biophysics
  • Super-resolution microscopy

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