Translation Microscopy (TRAM) for super-resolution imaging

TY - JOUR

T1 - Translation Microscopy (TRAM) for super-resolution imaging

AU - Qiu, Zhen

AU - Wilson, Rhodri S.

AU - Liu, Yuewei

AU - Dun, Alison R.

AU - Saleeb, Rebecca S.

AU - Liu, Dongsheng

AU - Rickman, Colin

AU - Frame, Margaret

AU - Duncan, Rory R.

AU - Lu, Weiping

N1 - Copyright: © 2016, The Author(s).

PY - 2016/1

Y1 - 2016/1

N2 - Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ‘ground-truth’ DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.

AB - Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ‘ground-truth’ DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.

KW - Computational biophysics

KW - Super-resolution microscopy

U2 - 10.1038/srep19993

DO - 10.1038/srep19993

M3 - Article

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 19993

ER -