Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis

Jeremy Minshull, J. Julian Blow, Tim Hunt

    Research output: Contribution to journalArticle

    272 Citations (Scopus)


    The cyclins are a family of proteins encoded by maternal mRNA. Cyclin polypeptides accumulate during interphase and are destroyed during mitosis at about the time of entry into anaphase. We show here that Xenopus oocytes contain mRNAs encoding two cyclins that are major translation products in a cell-free extract from activated eggs. Cutting these mRNAs with antisense oligonucleotides and endogenous RNAase H blocks entry into mitosis in a cell-free egg extract. The extracts can enter mitosis if either of the cyclin mRNAs is left intact. We conclude that the synthesis of these cyclins is necessary for mitotic cell cycles in cleaving Xenopus embryos.
    Original languageEnglish
    Pages (from-to)947-956
    Number of pages10
    Issue number6
    Publication statusPublished - 24 Mar 1989


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