Translation of Diverse Aramid- And 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro

Omer Ad, Kyle S. Hoffman, Andrew G. Cairns, Aaron L. Featherston, Scott J. Miller (Lead / Corresponding author), Dieter Söll (Lead / Corresponding author), Alanna Schepartz (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
9 Downloads (Pure)

Abstract

Here, we report that wild type Escherichia coli ribosomes accept and elongate precharged initiator tRNAs acylated with multiple benzoic acids, including aramid precursors, as well as malonyl (1,3-dicarbonyl) substrates to generate a diverse set of aramid-peptide and polyketide-peptide hybrid molecules. This work expands the scope of ribozyme- and ribosome-catalyzed chemical transformations, provides a starting point for in vivo translation engineering efforts, and offers an alternative strategy for the biosynthesis of polyketide-peptide natural products.

Original languageEnglish
Pages (from-to)1289-1294
Number of pages6
JournalACS Central Science
Volume5
Issue number7
Early online date26 Jun 2019
DOIs
Publication statusPublished - 24 Jul 2019

Fingerprint

Dive into the research topics of 'Translation of Diverse Aramid- And 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro'. Together they form a unique fingerprint.

Cite this