Translocation of carbon by Rhizoctonia solani in nutritionally-heterogeneous microcosms

Helen Jacobs, Graeme P. Boswell, Charles M. Scrimgeour, Fordyce A. Davidson, Geoffrey M. Gadd, Karl Ritz (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Responses of Rhizoctonia solani to spatial heterogeneity in sources of carbon, and associated translocation of carbon (C), were studied in a simple microcosm system comprising two discrete domains of agar gels separated on a glass slide and overlain with a porous membrane. Two arrangements of the gel pairs were used, one containing two equally large resources (representing 'homogeneous' conditions) and one containing a large and a negligible resource (representing 'heterogeneous' conditions). The nutrient sources were a standard mineral salt medium with or without glucose as sole C source. The fungus was inoculated onto one domain and growth responses determined by direct measurement of biomass. Translocation of C was quantified by use of 13C-enriched glucose. This substrate was either added to the agar at the outset, when studying newly developing colonies, or as a pulse into already established colonies. When growing in heterogeneous conditions, the fungus actively translocated C from a glucose-containing domain to sustain growth in the adjacent region lacking such a resource. In homogeneous conditions there was evidence of passive translocation (diffusion), but the fungus preferentially used local resource to maintain growth. Active translocation was only observed in newly growing colonies, whereas passive translocation occurred in both growing and established colonies. When the fungus was pulsed with a 13C-enriched glucose solution after 10 d growth, 2.5 times more 13C was taken up by the fungus grown in heterogeneous than homogeneous conditions, suggesting uptake exceeded local demands. In heterogeneous conditions, the total amount of 13C enriched glucose taken up by the fungus was independent of the location of the enriched glucose in the underlying medium. When the nylon membrane was replaced by Cellophane (an additional C source), degradation of the membrane and an increase in biomass occurred only in the heterogeneous system. The possible implications for these results in soil systems are discussed.

    Original languageEnglish
    Pages (from-to)453-462
    Number of pages10
    JournalMycological Research
    Volume108
    Issue number4
    DOIs
    Publication statusPublished - Apr 2004

    Fingerprint

    Rhizoctonia
    Thanatephorus cucumeris
    microcosm
    translocation
    glucose
    Fungi
    Carbon
    fungus
    Glucose
    fungi
    carbon
    resource
    Growth
    membrane
    agar
    Biomass
    Agar
    Membranes
    gel
    Gels

    Keywords

    • Biomass
    • Glucose
    • Rhizoctonia
    • Soil Microbiology

    Cite this

    Jacobs, Helen ; Boswell, Graeme P. ; Scrimgeour, Charles M. ; Davidson, Fordyce A. ; Gadd, Geoffrey M. ; Ritz, Karl. / Translocation of carbon by Rhizoctonia solani in nutritionally-heterogeneous microcosms. In: Mycological Research. 2004 ; Vol. 108, No. 4. pp. 453-462.
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    Translocation of carbon by Rhizoctonia solani in nutritionally-heterogeneous microcosms. / Jacobs, Helen; Boswell, Graeme P.; Scrimgeour, Charles M.; Davidson, Fordyce A.; Gadd, Geoffrey M.; Ritz, Karl (Lead / Corresponding author).

    In: Mycological Research, Vol. 108, No. 4, 04.2004, p. 453-462.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Jacobs, Helen

    AU - Boswell, Graeme P.

    AU - Scrimgeour, Charles M.

    AU - Davidson, Fordyce A.

    AU - Gadd, Geoffrey M.

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    AB - Responses of Rhizoctonia solani to spatial heterogeneity in sources of carbon, and associated translocation of carbon (C), were studied in a simple microcosm system comprising two discrete domains of agar gels separated on a glass slide and overlain with a porous membrane. Two arrangements of the gel pairs were used, one containing two equally large resources (representing 'homogeneous' conditions) and one containing a large and a negligible resource (representing 'heterogeneous' conditions). The nutrient sources were a standard mineral salt medium with or without glucose as sole C source. The fungus was inoculated onto one domain and growth responses determined by direct measurement of biomass. Translocation of C was quantified by use of 13C-enriched glucose. This substrate was either added to the agar at the outset, when studying newly developing colonies, or as a pulse into already established colonies. When growing in heterogeneous conditions, the fungus actively translocated C from a glucose-containing domain to sustain growth in the adjacent region lacking such a resource. In homogeneous conditions there was evidence of passive translocation (diffusion), but the fungus preferentially used local resource to maintain growth. Active translocation was only observed in newly growing colonies, whereas passive translocation occurred in both growing and established colonies. When the fungus was pulsed with a 13C-enriched glucose solution after 10 d growth, 2.5 times more 13C was taken up by the fungus grown in heterogeneous than homogeneous conditions, suggesting uptake exceeded local demands. In heterogeneous conditions, the total amount of 13C enriched glucose taken up by the fungus was independent of the location of the enriched glucose in the underlying medium. When the nylon membrane was replaced by Cellophane (an additional C source), degradation of the membrane and an increase in biomass occurred only in the heterogeneous system. The possible implications for these results in soil systems are discussed.

    KW - Biomass

    KW - Glucose

    KW - Rhizoctonia

    KW - Soil Microbiology

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