Transparent soil for imaging the rhizosphere

Helen Downie, Nicola Holden, Wilfred Otten, Andrew J. Spiers, Tracy A. Valentine, Lionel X. Dupuy

    Research output: Contribution to journalArticle

    96 Citations (Scopus)

    Abstract

    Understanding of soil processes is essential for addressing the global issues of food security, disease transmission and climate change. However, techniques for observing soil biology are lacking. We present a heterogeneous, porous, transparent substrate for in situ 3D imaging of living plants and root-associated microorganisms using particles of the transparent polymer, Nafion, and a solution with matching optical properties. Minerals and fluorescent dyes were adsorbed onto the Nafion particles for nutrient supply and imaging of pore size and geometry. Plant growth in transparent soil was similar to that in soil. We imaged colonization of lettuce roots by the human bacterial pathogen Escherichia coli O157:H7 showing micro-colony development. Micro-colonies may contribute to bacterial survival in soil. Transparent soil has applications in root biology, crop genetics and soil microbiology.
    Original languageEnglish
    Article numbere44276
    JournalPLoS ONE
    Volume7
    Issue number9
    DOIs
    Publication statusPublished - 11 Sep 2012

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