Trypanothione dependent peroxide metabolism in Crithidia fasciculata and Trypanosoma brucei

Graeme B. Henderson, Alan H. Fairlamb, Anthony Cerami

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    86 Citations (Scopus)

    Abstract

    A trypanothione-dependent peroxidase activity has been identified in the insect trypanosomatid Crithidia fasciculata and in the mammalian trypanosome Trypanosoma brucei. Using organic hydroperoxides as oxidant, specific peroxidase activities in these organisms are 5.0 and 1.0 nmol min-1 (108 cells)-1 respectively. The T. brucei peroxidase had an activity of 0.4 nmol min-1 (108 cells)-1 using hydrogen peroxide as oxidant. The enzyme is specific for the N1,N8-bis(glutathionyl)spermidine conjugate (dihydrotrypanothione); N1-mono-glutathionylspermidine is not a substrate. Experiments to demonstrate that this parasite peroxidase may contain selenium were inconclusive. However, bloodstream T. brucei can incorporate radiolabelled selenite into proteins.

    Original languageEnglish
    Pages (from-to)39-45
    Number of pages7
    JournalMolecular and Biochemical Parasitology
    Volume24
    Issue number1
    DOIs
    Publication statusPublished - May 1987

    Keywords

    • Crithidia fasciculata
    • Peroxide metabolism
    • Trypanosoma brucei
    • Trypanothione

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