Trypanothione dependent peroxide metabolism in Crithidia fasciculata and Trypanosoma brucei

Graeme B. Henderson; Alan H. Fairlamb; Anthony Cerami

doi:10.1016/0166-6851(87)90113-7

Trypanothione dependent peroxide metabolism in Crithidia fasciculata and Trypanosoma brucei

Graeme B. Henderson, Alan H. Fairlamb, Anthony Cerami

Research output: Contribution to journal › Article › peer-review

87 Citations (Scopus)

Abstract

A trypanothione-dependent peroxidase activity has been identified in the insect trypanosomatid Crithidia fasciculata and in the mammalian trypanosome Trypanosoma brucei. Using organic hydroperoxides as oxidant, specific peroxidase activities in these organisms are 5.0 and 1.0 nmol min^-1 (10⁸ cells)^-1 respectively. The T. brucei peroxidase had an activity of 0.4 nmol min^-1 (10⁸ cells)^-1 using hydrogen peroxide as oxidant. The enzyme is specific for the N¹,N⁸-bis(glutathionyl)spermidine conjugate (dihydrotrypanothione); N¹-mono-glutathionylspermidine is not a substrate. Experiments to demonstrate that this parasite peroxidase may contain selenium were inconclusive. However, bloodstream T. brucei can incorporate radiolabelled selenite into proteins.

Original language	English
Pages (from-to)	39-45
Number of pages	7
Journal	Molecular and Biochemical Parasitology
Volume	24
Issue number	1
DOIs	https://doi.org/10.1016/0166-6851(87)90113-7
Publication status	Published - May 1987

Keywords

Crithidia fasciculata
Peroxide metabolism
Trypanosoma brucei
Trypanothione

ASJC Scopus subject areas

Parasitology
Molecular Biology

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10.1016/0166-6851(87)90113-7

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@article{e072eff982194f48aa7d456bac3443cd,

title = "Trypanothione dependent peroxide metabolism in Crithidia fasciculata and Trypanosoma brucei",

abstract = "A trypanothione-dependent peroxidase activity has been identified in the insect trypanosomatid Crithidia fasciculata and in the mammalian trypanosome Trypanosoma brucei. Using organic hydroperoxides as oxidant, specific peroxidase activities in these organisms are 5.0 and 1.0 nmol min-1 (108 cells)-1 respectively. The T. brucei peroxidase had an activity of 0.4 nmol min-1 (108 cells)-1 using hydrogen peroxide as oxidant. The enzyme is specific for the N1,N8-bis(glutathionyl)spermidine conjugate (dihydrotrypanothione); N1-mono-glutathionylspermidine is not a substrate. Experiments to demonstrate that this parasite peroxidase may contain selenium were inconclusive. However, bloodstream T. brucei can incorporate radiolabelled selenite into proteins.",

keywords = "Crithidia fasciculata, Peroxide metabolism, Trypanosoma brucei, Trypanothione",

author = "Henderson, {Graeme B.} and Fairlamb, {Alan H.} and Anthony Cerami",

note = "Funding Information: The authors thank Ms. Helen Shim for technical assistance and Dr. Harvey Cohen (Rochester University) for helpful discussion. This work was supported by a grant from the Rockefeller Foundation (RF 85078, #127), grants from the National Institutes of Allergy and Infectious Diseases (AI 21429 and AI 19428) and by the UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases.",

year = "1987",

month = may,

doi = "10.1016/0166-6851(87)90113-7",

language = "English",

volume = "24",

pages = "39--45",

journal = "Molecular and Biochemical Parasitology",

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publisher = "Elsevier",

number = "1",

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T1 - Trypanothione dependent peroxide metabolism in Crithidia fasciculata and Trypanosoma brucei

AU - Henderson, Graeme B.

AU - Fairlamb, Alan H.

AU - Cerami, Anthony

N1 - Funding Information: The authors thank Ms. Helen Shim for technical assistance and Dr. Harvey Cohen (Rochester University) for helpful discussion. This work was supported by a grant from the Rockefeller Foundation (RF 85078, #127), grants from the National Institutes of Allergy and Infectious Diseases (AI 21429 and AI 19428) and by the UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases.

PY - 1987/5

Y1 - 1987/5

N2 - A trypanothione-dependent peroxidase activity has been identified in the insect trypanosomatid Crithidia fasciculata and in the mammalian trypanosome Trypanosoma brucei. Using organic hydroperoxides as oxidant, specific peroxidase activities in these organisms are 5.0 and 1.0 nmol min-1 (108 cells)-1 respectively. The T. brucei peroxidase had an activity of 0.4 nmol min-1 (108 cells)-1 using hydrogen peroxide as oxidant. The enzyme is specific for the N1,N8-bis(glutathionyl)spermidine conjugate (dihydrotrypanothione); N1-mono-glutathionylspermidine is not a substrate. Experiments to demonstrate that this parasite peroxidase may contain selenium were inconclusive. However, bloodstream T. brucei can incorporate radiolabelled selenite into proteins.

AB - A trypanothione-dependent peroxidase activity has been identified in the insect trypanosomatid Crithidia fasciculata and in the mammalian trypanosome Trypanosoma brucei. Using organic hydroperoxides as oxidant, specific peroxidase activities in these organisms are 5.0 and 1.0 nmol min-1 (108 cells)-1 respectively. The T. brucei peroxidase had an activity of 0.4 nmol min-1 (108 cells)-1 using hydrogen peroxide as oxidant. The enzyme is specific for the N1,N8-bis(glutathionyl)spermidine conjugate (dihydrotrypanothione); N1-mono-glutathionylspermidine is not a substrate. Experiments to demonstrate that this parasite peroxidase may contain selenium were inconclusive. However, bloodstream T. brucei can incorporate radiolabelled selenite into proteins.

KW - Crithidia fasciculata

KW - Peroxide metabolism

KW - Trypanosoma brucei

KW - Trypanothione

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U2 - 10.1016/0166-6851(87)90113-7

DO - 10.1016/0166-6851(87)90113-7

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C2 - 3614271

AN - SCOPUS:0023263401

SN - 0166-6851

VL - 24

SP - 39

EP - 45

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 1

ER -

Trypanothione dependent peroxide metabolism in Crithidia fasciculata and Trypanosoma brucei

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