Tumor Necrosis Factor-mediated survival of CD169+ cells promotes immune activation during vesicular stomatitis virus infection

Prashant V. Shinde, Haifeng C. Xu, Sathish Kumar Maney, Andreas Kloetgen, Sukumar Namineni, Yuan Zhuang, Nadine Honke, Namir Shaabani, Nicolas Bellora, Mareike Doerrenberg, Mirko Trilling, Vitaly I. Pozdeev, Nico van Rooijen, Stefanie Scheu, Klaus Pfeffer, Paul R. Crocker, Masato Tanaka, Sujitha Duggimpudi, Percy Knolle, Mathias HeikenwalderJürgen Ruland, Tak W. Mak, Dirk Brenner, Aleksandra A. Pandyra, Jessica I. Hoell, Arndt Borkhardt, Dieter Häussinger, Karl S. Lang, Philipp A. Lang

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Abstract

Innate immune activation is essential to mount an effective antiviral response and to prime adaptive immunity. Although a crucial role of CD169(+) cells during vesicular stomatitis virus (VSV) infections is increasingly recognized, factors regulating CD169(+) cells during viral infections remain unclear. Here we show that tumor necrosis factor is produced by CD11b(+) Ly6C(+)Ly6G(+) cells following infection with VSV. The absence of TNF or TNF receptor 1 (TNFR1) resulted in reduced numbers of CD169(+) cells and in reduced IFN-I production during VSV infection, with a severe disease outcome. Specifically, TNF triggered RelA translocation into the nucleus of CD169(+) cells; this translocation was inhibited when paracaspase MALT-1 was absent. Consequently, MALT1 deficiency resulted in reduced VSV replication, defective innate immune activation, and severe disease development. These findings indicate that TNF mediates the maintenance of CD169(+) cells and innate and adaptive immune activation during VSV infection.IMPORTANCE Over the last decade, strategically placed CD169(+) metallophilic macrophages in the marginal zone of the murine spleen and LN have been shown to play a very important role in host defense against viral pathogens. CD169(+) macrophages are shown to activate innate and adaptive immunity via "enforced virus replication" a controlled amplification of virus particles. However, factors regulating the CD169(+) macrophages remain to be studied. In this paper, we show that after Vesicular stomatitis virus infection, phagocytes produce tumor necrosis factor (TNF) which signals via TNFR1 and promote "enforced virus replication" in CD169(+) macrophages. Consequently, lack of TNF or TNFR1 resulted in defective immune activation and VSV clearance.

Original languageEnglish
Article numbere01637-17
Pages (from-to)1-18
Number of pages18
JournalJournal of Virology
Volume92
Issue number3
Early online date15 Nov 2017
DOIs
Publication statusPublished - Feb 2018

Keywords

  • TNF
  • MALT1
  • Innate immunity
  • Interferon
  • NF-κB
  • Interferons
  • Tumor necrosis factor
  • Adaptive Immunity
  • Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein/genetics
  • Transcription Factor RelA/metabolism
  • Mice, Inbred C57BL
  • Vesiculovirus/physiology
  • Macrophages/immunology
  • Immunity, Innate
  • Tumor Necrosis Factor-alpha/immunology
  • Animals
  • Virus Replication
  • Vesicular Stomatitis/immunology
  • Interferon Type I/immunology
  • Mice
  • Receptors, Tumor Necrosis Factor, Type I/immunology
  • Sialic Acid Binding Ig-like Lectin 1

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  • Cite this

    Shinde, P. V., Xu, H. C., Maney, S. K., Kloetgen, A., Namineni, S., Zhuang, Y., Honke, N., Shaabani, N., Bellora, N., Doerrenberg, M., Trilling, M., Pozdeev, V. I., van Rooijen, N., Scheu, S., Pfeffer, K., Crocker, P. R., Tanaka, M., Duggimpudi, S., Knolle, P., ... Lang, P. A. (2018). Tumor Necrosis Factor-mediated survival of CD169+ cells promotes immune activation during vesicular stomatitis virus infection. Journal of Virology, 92(3), 1-18. [e01637-17]. https://doi.org/10.1128/JVI.01637-17