TY - JOUR
T1 - Two distinct arginine methyltransferases are required for biogenesis of Sm-class ribonucleoproteins
AU - Gonsalvez, Graydon B.
AU - Tian, Liping
AU - Ospina, Jason K.
AU - Boisvert, François Michel
AU - Lamond, Angus I.
AU - Matera, A. Gregory
PY - 2007/8/27
Y1 - 2007/8/27
N2 - Small nuclear ribonucleoproteins (snRNPs) are core components of the spliceosome. The U1, U2, U4, and U5 snRNPs each contain a common set of seven Sm proteins. Three of these Sm proteins are posttranslationally modified to contain symmetric dimethylarginine (sDMA) residues within their C-terminal tails. However, the precise function of this modification in the snRNP biogenesis pathway is unclear. Several lines of evidence suggest that the methyltransferase protein arginine methyltransferase 5 (PRMT5) is responsible for sDMA modification of Sm proteins. We found that in human cells, PRMT5 and a newly discovered type II methyltransferase, PRMT7, are each required for Sm protein sDMA modification. Furthermore, we show that the two enzymes function nonredundantly in Sm protein methylation. Lastly, we provide in vivo evidence demonstrating that Sm protein sDMA modification is required for snRNP biogenesis in human cells.
AB - Small nuclear ribonucleoproteins (snRNPs) are core components of the spliceosome. The U1, U2, U4, and U5 snRNPs each contain a common set of seven Sm proteins. Three of these Sm proteins are posttranslationally modified to contain symmetric dimethylarginine (sDMA) residues within their C-terminal tails. However, the precise function of this modification in the snRNP biogenesis pathway is unclear. Several lines of evidence suggest that the methyltransferase protein arginine methyltransferase 5 (PRMT5) is responsible for sDMA modification of Sm proteins. We found that in human cells, PRMT5 and a newly discovered type II methyltransferase, PRMT7, are each required for Sm protein sDMA modification. Furthermore, we show that the two enzymes function nonredundantly in Sm protein methylation. Lastly, we provide in vivo evidence demonstrating that Sm protein sDMA modification is required for snRNP biogenesis in human cells.
UR - http://www.scopus.com/inward/record.url?scp=34548356376&partnerID=8YFLogxK
U2 - 10.1083/jcb.200702147
DO - 10.1083/jcb.200702147
M3 - Article
C2 - 17709427
AN - SCOPUS:34548356376
SN - 0021-9525
VL - 178
SP - 733
EP - 740
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -