Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin

ANDREW R BUTLER, JOHN H WHITE, YETUNDE FOLAWIYO, ANGELA EDLIN, DONALD GARDINER, MICHAEL J. R. STARK

    Research output: Contribution to journalArticlepeer-review

    75 Citations (Scopus)

    Abstract

    The Kluyveromyces lactis toxin causes an arrest of sensitive yeast cells in the G1 phase of the cell division cycle. Two complementary genetic approaches have been undertaken in the yeast Saccharomyces cerevisiae to understand the mode of action of this toxin. First, two sequences conferring toxin resistance specifically in high copy number have been isolated and shown to encode a tRNA3Glu and a novel polypeptide. Disruption of the latter sequence in the yeast genome conferred toxin resistance and revealed that it was nonessential, chile the effect of the tRNA3Glu was highly specific and mediated resistance by affecting the toxin's target. An alpha-specific, copy number-independent suppressor of toxin sensitivity was also isolated and identified as MATa, consistent with the observation that diploid cells are partially resistant to the toxin. Second, in a comprehensive screen for toxin-resistant mutants, representatives of 13 complementation groups have been obtained and characterized to determine whether they are altered in the toxin's intracellular target. Of 10 genes found to affect the target process, one (KT112) was found to encode the novel polypeptide previously identified as a multicopy resistance determinant. Thus, both loss of KT112 function and elevated KT112 copy number can cause resistance to the and K. lactis toxin.

    Original languageEnglish
    Pages (from-to)6306-6316
    Number of pages11
    JournalMolecular and Cellular Biology
    Volume14
    Issue number9
    Publication statusPublished - Sep 1994

    Cite this