Two-photon ablation with 1278 nm laser radiation

Pascal Fischer; Alan McWilliam; Lynn Paterson; Christian T.A. Brown; Wilson Sibbett; Kishan Dholakia; Michael P. MacDonald

doi:10.1088/1464-4258/9/6/S04

Two-photon ablation with 1278 nm laser radiation

Pascal Fischer, Alan McWilliam, Lynn Paterson, Christian T.A. Brown, Wilson Sibbett, Kishan Dholakia, Michael P. MacDonald

Physics

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We report on precise two-photon ablation with 110fs laser pulses at 1278nm, emitted from a Cr:forsterite laser. Selective two-photon ablation of Muntjac deer chromosomes is demonstrated. The two-photon absorption at 639nm was enhanced by using Methylene blue dye as a photosensitizer. This stain has a strong absorption in the region around 650nm but 100% transmission around 1300nm, allowing increased specificity: material that has absorbed the dye is ablated but undyed material is left unaffected. The low group velocity dispersion in glass at 1278nm led to negligible pulse broadening in the focusing objective where the 100fs pulses stretched to 110fs. This contrasts to the 100fs pulses at 780nm that were measured to stretch to 300fs under the same conditions.

Original language	English
Article number	S04
Journal	Journal of Optics A: Pure and Applied Optics
Volume	9
Issue number	6
DOIs	https://doi.org/10.1088/1464-4258/9/6/S04
Publication status	Published - 1 Jun 2007

Keywords

Biological and medical applications
Chromosomes
Nonlinear optics
Optical radiation (near ultraviolet, visible, and infrared)

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

Access to Document

10.1088/1464-4258/9/6/S04

Cite this

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title = "Two-photon ablation with 1278 nm laser radiation",

abstract = "We report on precise two-photon ablation with 110fs laser pulses at 1278nm, emitted from a Cr:forsterite laser. Selective two-photon ablation of Muntjac deer chromosomes is demonstrated. The two-photon absorption at 639nm was enhanced by using Methylene blue dye as a photosensitizer. This stain has a strong absorption in the region around 650nm but 100% transmission around 1300nm, allowing increased specificity: material that has absorbed the dye is ablated but undyed material is left unaffected. The low group velocity dispersion in glass at 1278nm led to negligible pulse broadening in the focusing objective where the 100fs pulses stretched to 110fs. This contrasts to the 100fs pulses at 780nm that were measured to stretch to 300fs under the same conditions.",

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T1 - Two-photon ablation with 1278 nm laser radiation

AU - Fischer, Pascal

AU - McWilliam, Alan

AU - Paterson, Lynn

AU - Brown, Christian T.A.

AU - Sibbett, Wilson

AU - Dholakia, Kishan

AU - MacDonald, Michael P.

PY - 2007/6/1

Y1 - 2007/6/1

N2 - We report on precise two-photon ablation with 110fs laser pulses at 1278nm, emitted from a Cr:forsterite laser. Selective two-photon ablation of Muntjac deer chromosomes is demonstrated. The two-photon absorption at 639nm was enhanced by using Methylene blue dye as a photosensitizer. This stain has a strong absorption in the region around 650nm but 100% transmission around 1300nm, allowing increased specificity: material that has absorbed the dye is ablated but undyed material is left unaffected. The low group velocity dispersion in glass at 1278nm led to negligible pulse broadening in the focusing objective where the 100fs pulses stretched to 110fs. This contrasts to the 100fs pulses at 780nm that were measured to stretch to 300fs under the same conditions.

AB - We report on precise two-photon ablation with 110fs laser pulses at 1278nm, emitted from a Cr:forsterite laser. Selective two-photon ablation of Muntjac deer chromosomes is demonstrated. The two-photon absorption at 639nm was enhanced by using Methylene blue dye as a photosensitizer. This stain has a strong absorption in the region around 650nm but 100% transmission around 1300nm, allowing increased specificity: material that has absorbed the dye is ablated but undyed material is left unaffected. The low group velocity dispersion in glass at 1278nm led to negligible pulse broadening in the focusing objective where the 100fs pulses stretched to 110fs. This contrasts to the 100fs pulses at 780nm that were measured to stretch to 300fs under the same conditions.

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KW - Chromosomes

KW - Nonlinear optics

KW - Optical radiation (near ultraviolet, visible, and infrared)

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Two-photon ablation with 1278 nm laser radiation

Abstract

Keywords

ASJC Scopus subject areas

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