Two zinc finger proteins with functions in m6A writing interact with HAKAI

Mi Zhang; Zsuzsanna Bodi; Katarzyna Mackinnon; Silin Zhong; Nathan Archer; Nigel P. Mongan; Gordon G. Simpson; Rupert G. Fray

doi:10.1038/s41467-022-28753-3

Two zinc finger proteins with functions in m6A writing interact with HAKAI

Mi Zhang, Zsuzsanna Bodi, Katarzyna Mackinnon, Silin Zhong, Nathan Archer, Nigel P. Mongan, Gordon G. Simpson, Rupert G. Fray (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

101 Downloads (Pure)

Abstract

The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

Original language	English
Article number	1127
Number of pages	15
Journal	Nature Communications
Volume	13
DOIs	https://doi.org/10.1038/s41467-022-28753-3
Publication status	Published - 2 Mar 2022

Keywords

Plant development
Plant molecular biology
RNA modification

ASJC Scopus subject areas

General Physics and Astronomy
General Chemistry
General Biochemistry,Genetics and Molecular Biology

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10.1038/s41467-022-28753-3Licence: CC BY

Final Published VersionFinal published version, 6.92 MBLicence: CC BY

Cite this

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title = "Two zinc finger proteins with functions in m6A writing interact with HAKAI",

abstract = "The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.",

keywords = "Plant development, Plant molecular biology, RNA modification",

author = "Mi Zhang and Zsuzsanna Bodi and Katarzyna Mackinnon and Silin Zhong and Nathan Archer and Mongan, {Nigel P.} and Simpson, {Gordon G.} and Fray, {Rupert G.}",

note = "Funding for this research was provided by: China Scholarship Council University of Nottingham Future Food Beacon Doctoral/Post-Doctoral Prize RCUK | Biotechnology and Biological Sciences Research Council (BB/M010066/1, BB/M008606/1, BB/S006478/1) {\textcopyright} 2022. The Author(s).",

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doi = "10.1038/s41467-022-28753-3",

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AU - Zhang, Mi

AU - Bodi, Zsuzsanna

AU - Mackinnon, Katarzyna

AU - Zhong, Silin

AU - Archer, Nathan

AU - Mongan, Nigel P.

AU - Simpson, Gordon G.

AU - Fray, Rupert G.

N1 - Funding for this research was provided by: China Scholarship Council University of Nottingham Future Food Beacon Doctoral/Post-Doctoral Prize RCUK | Biotechnology and Biological Sciences Research Council (BB/M010066/1, BB/M008606/1, BB/S006478/1) © 2022. The Author(s).

PY - 2022/3/2

Y1 - 2022/3/2

N2 - The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

AB - The methyltransferase complex (m6A writer), which catalyzes the deposition of N6-methyladenosine (m6A) in mRNAs, is highly conserved across most eukaryotic organisms, but its components and interactions between them are still far from fully understood. Here, using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, are discovered as components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA (mRNA adenosine methylase A). Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation and decreased lateral root formation. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ2 is likely the plant equivalent of ZC3H13 (Flacc) of the metazoan m6A-METTL Associated Complex.

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Two zinc finger proteins with functions in m6A writing interact with HAKAI

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The Arabidopsis Epitranscriptome (Joint with University of Nottingham)

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Two zinc finger proteins with functions in m6A writing interact with HAKAI

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ASJC Scopus subject areas

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The Arabidopsis Epitranscriptome (Joint with University of Nottingham)

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