U12-dependent intron splicing in plants

C. G. Simpson, J. W. S. Brown

    Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

    7 Citations (Scopus)

    Abstract

    U12-dependent (U12) introns have persisted in the genomes of plants since the ancestral divergence between plants and metazoans. These introns, which are rare, are found in a range of genes that include essential functions in DNA replication and RNA metabolism and are implicated in regulating the expression of their host genes. U12 introns are removed from pre-mRNAs by a U12 intronspecific spliceosome. Although this spliceosome shares many properties with the more abundant U2-dependent (U2) intron spliceosome, four of the five small nuclear RNAs (snRNAs) required for splicing are different and specific for the unique splicing of U12 introns. Evidence in plants so far indicates that splicing signals of plant U12 introns and their splicing machinery are similar to U12 intron splicing in other eukaryotes. In addition to the high conservation of splicing signals, plant U12 introns also retain unique characteristic features of plant U2 introns, such as UA-richness, which suggests a requirement for plant-specific components for both the U2 and U12 splicing reaction. This chapter compares U12 and U2 splicing and reviews what is known about plant U12 introns and their possible role in gene expression.
    Original languageEnglish
    Title of host publicationNuclear pre-mRNA processing in plants
    EditorsAnireddy S. N. Reddy, Maxim Golovkin
    Place of PublicationBerlin
    PublisherSpringer
    Pages61-82
    Number of pages22
    ISBN (Electronic)9783540767763
    ISBN (Print)9783540767756
    DOIs
    Publication statusPublished - 2008

    Publication series

    NameCurrent topics in microbiology and immunology
    PublisherSpringer
    Volume326
    ISSN (Print)0070-217x

    Fingerprint

    introns
    spliceosomes
    small nuclear RNA
    RNA splicing
    DNA replication
    eukaryotic cells
    RNA
    gene expression
    metabolism
    genome

    Cite this

    Simpson, C. G., & Brown, J. W. S. (2008). U12-dependent intron splicing in plants. In A. S. N. Reddy, & M. Golovkin (Eds.), Nuclear pre-mRNA processing in plants (pp. 61-82). (Current topics in microbiology and immunology; Vol. 326). Berlin: Springer . https://doi.org/10.1007/978-3-540-76776-3_4
    Simpson, C. G. ; Brown, J. W. S. / U12-dependent intron splicing in plants. Nuclear pre-mRNA processing in plants. editor / Anireddy S. N. Reddy ; Maxim Golovkin. Berlin : Springer , 2008. pp. 61-82 (Current topics in microbiology and immunology).
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    Simpson, CG & Brown, JWS 2008, U12-dependent intron splicing in plants. in ASN Reddy & M Golovkin (eds), Nuclear pre-mRNA processing in plants. Current topics in microbiology and immunology, vol. 326, Springer , Berlin, pp. 61-82. https://doi.org/10.1007/978-3-540-76776-3_4

    U12-dependent intron splicing in plants. / Simpson, C. G.; Brown, J. W. S.

    Nuclear pre-mRNA processing in plants. ed. / Anireddy S. N. Reddy; Maxim Golovkin. Berlin : Springer , 2008. p. 61-82 (Current topics in microbiology and immunology; Vol. 326).

    Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution

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    Simpson CG, Brown JWS. U12-dependent intron splicing in plants. In Reddy ASN, Golovkin M, editors, Nuclear pre-mRNA processing in plants. Berlin: Springer . 2008. p. 61-82. (Current topics in microbiology and immunology). https://doi.org/10.1007/978-3-540-76776-3_4