UBF activates RNA polymerase I transcription by stimulating promoter escape

Kostya I. Panov, J. Karsten Friedrich, Jacqueline Russell, Joost C. B. M. Zomerdijk

    Research output: Contribution to journalArticle

    66 Citations (Scopus)

    Abstract

    Ribosomal RNA gene transcription by RNA polymerase I (Pol I) is the driving force behind ribosome biogenesis, vital to cell growth and proliferation. The key activator of Pol I transcription, UBF, has been proposed to act by facilitating recruitment of Pol I and essential basal factor SL1 to rDNA promoters. However, we found no evidence that UBF could stimulate recruitment or stabilization of the pre-initiation complex (PIC) in reconstituted transcription assays. In this, UBF is fundamentally different from archetypal activators of transcription. Our data imply that UBF exerts its stimulatory effect on RNA synthesis, after PIC formation, promoter opening and first phosphodiester bond formation and before elongation. We provide evidence to suggest that UBF activates transcription in the transition between initiation and elongation, at promoter escape by Pol I. This novel role for UBF in promoter escape would allow control of rRNA synthesis at active rDNA repeats, independent of and complementary to the promoter-specific targeting of SL1 and Pol I during PIC assembly. We posit that stimulation of promoter escape could be a general mechanism of activator function.
    Original languageEnglish
    Pages (from-to)3310-3322
    Number of pages13
    JournalThe EMBO Journal
    Volume25
    Issue number14
    DOIs
    Publication statusPublished - 2006

    Keywords

    • Promoter escape
    • Recruitment
    • RNA polymerase I
    • Transcription activation
    • UBF

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