Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Ksenia Krupina; Charlotte Kleiss; Thibaud Metzger; Sadek Fournane; Stephane Schmucker; Kay Hofmann; Benoit Fischer; Nicodeme Paul; Iain Malcolm Porter; Wolfgang Raffelsberger; Olivier Poch; Jason Reese Swedlow; Laurent Brino; Izabela Sumara

doi:10.1016/j.devcel.2015.12.017

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Ksenia Krupina, Charlotte Kleiss, Thibaud Metzger, Sadek Fournane, Stephane Schmucker, Kay Hofmann, Benoit Fischer, Nicodeme Paul, Iain Malcolm Porter, Wolfgang Raffelsberger, Olivier Poch, Jason Reese Swedlow, Laurent Brino, Izabela Sumara (Lead / Corresponding author)

Gene Regulation and Expression

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

Original language	English
Pages (from-to)	63-78
Number of pages	16
Journal	Developmental Cell
Volume	36
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2015.12.017
Publication status	Published - 11 Jan 2016

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Krupina, Ksenia ; Kleiss, Charlotte ; Metzger, Thibaud ; Fournane, Sadek ; Schmucker, Stephane ; Hofmann, Kay ; Fischer, Benoit ; Paul, Nicodeme ; Porter, Iain Malcolm ; Raffelsberger, Wolfgang ; Poch, Olivier ; Swedlow, Jason Reese ; Brino, Laurent ; Sumara, Izabela. / Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules. In: Developmental Cell. 2016 ; Vol. 36, No. 1. pp. 63-78.

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abstract = "Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.",

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Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules. / Krupina, Ksenia; Kleiss, Charlotte; Metzger, Thibaud; Fournane, Sadek; Schmucker, Stephane; Hofmann, Kay; Fischer, Benoit; Paul, Nicodeme; Porter, Iain Malcolm; Raffelsberger, Wolfgang; Poch, Olivier; Swedlow, Jason Reese; Brino, Laurent; Sumara, Izabela (Lead / Corresponding author).

In: Developmental Cell, Vol. 36, No. 1, 11.01.2016, p. 63-78.

Research output: Contribution to journal › Article › peer-review

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AU - Krupina, Ksenia

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AU - Schmucker, Stephane

AU - Hofmann, Kay

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AU - Paul, Nicodeme

AU - Porter, Iain Malcolm

AU - Raffelsberger, Wolfgang

AU - Poch, Olivier

AU - Swedlow, Jason Reese

AU - Brino, Laurent

AU - Sumara, Izabela

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