Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Ksenia Krupina, Charlotte Kleiss, Thibaud Metzger, Sadek Fournane, Stephane Schmucker, Kay Hofmann, Benoit Fischer, Nicodeme Paul, Iain Malcolm Porter, Wolfgang Raffelsberger, Olivier Poch, Jason Reese Swedlow, Laurent Brino, Izabela Sumara (Lead / Corresponding author)

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18 Citations (Scopus)

Abstract

Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

Original languageEnglish
Pages (from-to)63-78
Number of pages16
JournalDevelopmental Cell
Volume36
Issue number1
DOIs
Publication statusPublished - 11 Jan 2016

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Krupina, K., Kleiss, C., Metzger, T., Fournane, S., Schmucker, S., Hofmann, K., Fischer, B., Paul, N., Porter, I. M., Raffelsberger, W., Poch, O., Swedlow, J. R., Brino, L., & Sumara, I. (2016). Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules. Developmental Cell, 36(1), 63-78. https://doi.org/10.1016/j.devcel.2015.12.017