TY - JOUR
T1 - Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules
AU - Krupina, Ksenia
AU - Kleiss, Charlotte
AU - Metzger, Thibaud
AU - Fournane, Sadek
AU - Schmucker, Stephane
AU - Hofmann, Kay
AU - Fischer, Benoit
AU - Paul, Nicodeme
AU - Porter, Iain Malcolm
AU - Raffelsberger, Wolfgang
AU - Poch, Olivier
AU - Swedlow, Jason Reese
AU - Brino, Laurent
AU - Sumara, Izabela
PY - 2016/1/11
Y1 - 2016/1/11
N2 - Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.
AB - Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.
UR - http://www.scopus.com/inward/record.url?scp=84958744402&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2015.12.017
DO - 10.1016/j.devcel.2015.12.017
M3 - Article
C2 - 26766443
AN - SCOPUS:84958744402
VL - 36
SP - 63
EP - 78
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 1
ER -