Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Ksenia Krupina; Charlotte Kleiss; Thibaud Metzger; Sadek Fournane; Stephane Schmucker; Kay Hofmann; Benoit Fischer; Nicodeme Paul; Iain Malcolm Porter; Wolfgang Raffelsberger; Olivier Poch; Jason Reese Swedlow; Laurent Brino; Izabela Sumara

doi:10.1016/j.devcel.2015.12.017

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Ksenia Krupina, Charlotte Kleiss, Thibaud Metzger, Sadek Fournane, Stephane Schmucker, Kay Hofmann, Benoit Fischer, Nicodeme Paul, Iain Malcolm Porter, Wolfgang Raffelsberger, Olivier Poch, Jason Reese Swedlow, Laurent Brino, Izabela Sumara (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

Original language	English
Pages (from-to)	63-78
Number of pages	16
Journal	Developmental Cell
Volume	36
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2015.12.017
Publication status	Published - 11 Jan 2016

ASJC Scopus subject areas

Developmental Biology

Access to Document

10.1016/j.devcel.2015.12.017Licence: Elsevier User Licence

Cite this

@article{0c5bb699636441b3a1b19a5d38ed1b6a,

title = "Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules",

abstract = "Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.",

author = "Ksenia Krupina and Charlotte Kleiss and Thibaud Metzger and Sadek Fournane and Stephane Schmucker and Kay Hofmann and Benoit Fischer and Nicodeme Paul and Porter, {Iain Malcolm} and Wolfgang Raffelsberger and Olivier Poch and Swedlow, {Jason Reese} and Laurent Brino and Izabela Sumara",

year = "2016",

month = jan,

day = "11",

doi = "10.1016/j.devcel.2015.12.017",

language = "English",

volume = "36",

pages = "63--78",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

AU - Krupina, Ksenia

AU - Kleiss, Charlotte

AU - Metzger, Thibaud

AU - Fournane, Sadek

AU - Schmucker, Stephane

AU - Hofmann, Kay

AU - Fischer, Benoit

AU - Paul, Nicodeme

AU - Porter, Iain Malcolm

AU - Raffelsberger, Wolfgang

AU - Poch, Olivier

AU - Swedlow, Jason Reese

AU - Brino, Laurent

AU - Sumara, Izabela

PY - 2016/1/11

Y1 - 2016/1/11

N2 - Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

AB - Mitosis ensures equal segregation of the genome and is controlled by a variety of ubiquitylation signals on substrate proteins. However, it remains unexplored how the versatile ubiquitin code is read out during mitotic progression. Here, we identify the ubiquitin receptor protein UBASH3B as an important regulator of mitosis. UBASH3B interacts with ubiquitylated Aurora B, one of the main kinases regulating chromosome segregation, and controls its subcellular localization but not protein levels. UBASH3B is a limiting factor in this pathway and is sufficient to localize Aurora B to microtubules prior to anaphase. Importantly, targeting Aurora B to microtubules by UBASH3B is necessary for the timing and fidelity of chromosome segregation in human cells. Our findings uncover an important mechanism defining how ubiquitin attachment to a substrate protein is decoded during mitosis.

UR - http://www.scopus.com/inward/record.url?scp=84958744402&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2015.12.017

DO - 10.1016/j.devcel.2015.12.017

M3 - Article

C2 - 26766443

AN - SCOPUS:84958744402

SN - 1534-5807

VL - 36

SP - 63

EP - 78

JO - Developmental Cell

JF - Developmental Cell

IS - 1

ER -

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

The Open Microscopy Environment: Image Informatics for Biological Sciences (Joint with Imperial College, Oxford University, Institut Pasteur, Carnegie-Mellon University, University of Wisconsin, Harvard Medical School & University of Edinburgh)

Cite this

Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

The Open Microscopy Environment: Image Informatics for Biological Sciences (Joint with Imperial College, Oxford University, Institut Pasteur, Carnegie-Mellon University, University of Wisconsin, Harvard Medical School & University of Edinburgh)

Cite this