Abstract
Ultrasound-mediated targeted drug delivery has been a subject for a dedicated research activity for several decades. Nevertheless, in vitro studies in this field of research are characterized by their inconsistencies. To improve the repeatability of such experiments, a novel approach of multifocal spot generation was investigated. A multifocal pattern of 16 spots was utilized using an iterative Gerchberg-Saxton algorithm. The pattern was applied to insonate a 96-well Petri dish plate using a clinically available planar-phased array transducer with approximately 1000 elements with a central frequency of 0.55 MHz. The pattern was acoustically characterized and applied to a monolayer of human breast cancer cell line in the 96-well plate. With the help of ultrasonic contrast agents, the intracellular drug uptake was increased by an average factor of 3.5 compared with the control group. (E-mail: [email protected]) (C) 2013 World Federation for Ultrasound in Medicine & Biology.
Original language | English |
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Pages (from-to) | 507-514 |
Number of pages | 8 |
Journal | Ultrasound in Medicine and Biology |
Volume | 39 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- Focused ultrasound
- ASSAY
- GENE DELIVERY
- CELLS
- Multifocus beam patterns
- CHEMOSENSITIVITY
- Microbubbles
- Cancer cell lines
- Targeted drug delivery