Ultrasound mediated transfection of HT29 colorectal cancer cells in vitro: Preliminary results

R. Hart, L. Newman

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    HT29 human colorectal cancer cells, from an immortal cell line, were grown to confluence on four 11 mm coverslips in minimal essential culture medium using 60 mm culture dishes. One dish was kept as a control. Following washing with serum-free medium, 1 mg of fluorescein isothiocyanate (FITC) conjugated with bovine serum albumin (BSA) was added to the remaining three dishes. FITC-BSA was added to dish 2. Sterile gas-filled microspheres (average diameter 3 μm) were added to dishes 3 and 4, in an approximate ratio of 230 microspheres to each cell. Dish 4 was aseptically insonated using 2.5 MHz pulsed wave diagnostic spectral Doppler ultrasound for 5 minutes. All cultures were then on-grown for a further 24 hours in minimal essential culture medium. Subsequently they were washed and dried with methanol, mounted using DPX medium and microscopically viewed. Dishes one, two and three showed low levels of background fluorescence, while dish four showed strong cellular fluorescence. The presence of microspheres during insonation facilitates the non-lethal entry of a large molecule (FITC conjugated BSA) into HT29 colorectal cancer cells.

    Original languageEnglish
    Title of host publicationANZIIS 2001 - Proceedings of the 7th Australian and New Zealand Intelligent Information Systems Conference
    PublisherInstitute of Electrical and Electronics Engineers
    Pages439-442
    Number of pages4
    ISBN (Print)1740520610, 9781740520614
    DOIs
    Publication statusPublished - Nov 2001
    Event7th Australian and New Zealand Intelligent Information Systems Conference, ANZIIS 2001 - Perth, Australia
    Duration: 18 Nov 200121 Nov 2001

    Conference

    Conference7th Australian and New Zealand Intelligent Information Systems Conference, ANZIIS 2001
    CountryAustralia
    CityPerth
    Period18/11/0121/11/01

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  • Cite this

    Hart, R., & Newman, L. (2001). Ultrasound mediated transfection of HT29 colorectal cancer cells in vitro: Preliminary results. In ANZIIS 2001 - Proceedings of the 7th Australian and New Zealand Intelligent Information Systems Conference (pp. 439-442). [974119] Institute of Electrical and Electronics Engineers. https://doi.org/10.1109/ANZIIS.2001.974119