Ultrastructure of COPII vesicle formation in yeast characterized by correlative light and electron microscopy

Alejandro Melero (Lead / Corresponding author), Jerome Boulanger, Wanda Kukulski, Elizabeth A Miller (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
10 Downloads (Pure)


Traffic of proteins out of the endoplasmic reticulum (ER) is driven by the COPII coat, a layered protein scaffold that mediates the capture of cargo proteins and the remodeling of the ER membrane into spherical vesicular carriers. Although the components of this machinery have been genetically defined, and the mechanisms of coat assembly extensively explored in vitro, understanding the physical mechanisms of membrane remodeling in cells remains a challenge. Here we use correlative light and electron microscopy (CLEM) to visualize the nanoscale ultrastructure of membrane remodeling at ER exit sites (ERES) in yeast cells. Using various COPII mutants, we have determined the broad contribution that each layer of the coat makes to membrane remodeling. Our data suggest that inner coat components define the radius of curvature, whereas outer coat components facilitate membrane fission. The organization of the coat in conjunction with membrane biophysical properties determines the ultrastructure of vesicles and thus the efficiency of protein transport.

Original languageEnglish
Article numberar122
Number of pages11
JournalMolecular Biology of the Cell
Issue number13
Early online date13 Oct 2022
Publication statusPublished - 1 Nov 2022


  • COP-Coated Vesicles/metabolism
  • Endoplasmic Reticulum/metabolism
  • Golgi Apparatus/metabolism
  • Microscopy, Electron
  • Protein Transport
  • Proteins/metabolism
  • Saccharomyces cerevisiae/metabolism


Dive into the research topics of 'Ultrastructure of COPII vesicle formation in yeast characterized by correlative light and electron microscopy'. Together they form a unique fingerprint.

Cite this