TY - JOUR
T1 - Unlocking the barley genome by chromosomal and comparative genomics
AU - Mayer, Klaus F.X.
AU - Martis, Mihaela
AU - Hedley, Pete E.
AU - Šimková, Hana
AU - Liu, Hui
AU - Morris, Jenny A.
AU - Steuernagel, Burkhard
AU - Taudien, Stefan
AU - Roessner, Stephan
AU - Gundlach, Heidrun
AU - Kubaláková, Marie
AU - Suchánková, Pavla
AU - Murat, Florent
AU - Felder, Marius
AU - Nussbaumer, Thomas
AU - Graner, Andreas
AU - Salse, Jerome
AU - Endo, Takashi
AU - Sakai, Hiroaki
AU - Tanaka, Tsuyoshi
AU - Itoh, Takeshi
AU - Sato, Kazuhiro
AU - Platzer, Matthias
AU - Matsumoto, Takashi
AU - Scholz, Uwe
AU - Doležel, Jaroslav
AU - Waugh, Robbie
AU - Stein, Nils
PY - 2011/4/1
Y1 - 2011/4/1
N2 - We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign;86% of the estimated;32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
AB - We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign;86% of the estimated;32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.
UR - http://www.scopus.com/inward/record.url?scp=79957676864&partnerID=8YFLogxK
U2 - 10.1105/tpc.110.082537
DO - 10.1105/tpc.110.082537
M3 - Article
C2 - 21467582
AN - SCOPUS:79957676864
SN - 1040-4651
VL - 23
SP - 1249
EP - 1263
JO - The Plant Cell
JF - The Plant Cell
IS - 4
ER -