Unlocking the barley genome by chromosomal and comparative genomics

TY - JOUR

T1 - Unlocking the barley genome by chromosomal and comparative genomics

AU - Mayer, Klaus F.X.

AU - Martis, Mihaela

AU - Hedley, Pete E.

AU - Šimková, Hana

AU - Liu, Hui

AU - Morris, Jenny A.

AU - Steuernagel, Burkhard

AU - Taudien, Stefan

AU - Roessner, Stephan

AU - Gundlach, Heidrun

AU - Kubaláková, Marie

AU - Suchánková, Pavla

AU - Murat, Florent

AU - Felder, Marius

AU - Nussbaumer, Thomas

AU - Graner, Andreas

AU - Salse, Jerome

AU - Endo, Takashi

AU - Sakai, Hiroaki

AU - Tanaka, Tsuyoshi

AU - Itoh, Takeshi

AU - Sato, Kazuhiro

AU - Platzer, Matthias

AU - Matsumoto, Takashi

AU - Scholz, Uwe

AU - Doležel, Jaroslav

AU - Waugh, Robbie

AU - Stein, Nils

PY - 2011/4/1

Y1 - 2011/4/1

N2 - We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign;86% of the estimated;32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.

AB - We used a novel approach that incorporated chromosome sorting, next-generation sequencing, array hybridization, and systematic exploitation of conserved synteny with model grasses to assign;86% of the estimated;32,000 barley (Hordeum vulgare) genes to individual chromosome arms. Using a series of bioinformatically constructed genome zippers that integrate gene indices of rice (Oryza sativa), sorghum (Sorghum bicolor), and Brachypodium distachyon in a conserved synteny model, we were able to assemble 21,766 barley genes in a putative linear order. We show that the barley (H) genome displays a mosaic of structural similarity to hexaploid bread wheat (Triticum aestivum) A, B, and D subgenomes and that orthologous genes in different grasses exhibit signatures of positive selection in different lineages. We present an ordered, information-rich scaffold of the barley genome that provides a valuable and robust framework for the development of novel strategies in cereal breeding.

UR - https://www.scopus.com/pages/publications/79957676864

U2 - 10.1105/tpc.110.082537

DO - 10.1105/tpc.110.082537

M3 - Article

C2 - 21467582

AN - SCOPUS:79957676864

SN - 1040-4651

VL - 23

SP - 1249

EP - 1263

JO - The Plant Cell

JF - The Plant Cell

IS - 4

ER -