Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo

Valerie Stock, Linda Böhmert (Lead / Corresponding author), Elisa Lisicki, Rafael Block, Julia Cara-Carmona, Laura Kim Pack, Regina Selb, Dajana Lichtenstein, Linn Voss, Colin J. Henderson, Elke Zabinsky, Holger Sieg, Albert Braeuning, Alfonso Lampen

Research output: Contribution to journalArticle

Abstract

Evidence exists that humans are exposed to plastic microparticles via diet. Data on intestinal particle uptake and health-related effects resulting from microplastic exposure are scarce. Aim of the study was to analyze the uptake and effects of microplastic particles in human in vitro systems and in rodents in vivo. The gastrointestinal uptake of microplastics was studied in vitro using the human intestinal epithelial cell line Caco-2 and thereof-derived co-cultures mimicking intestinal M-cells and goblet cells. Different sizes of spherical fluorescent polystyrene (PS) particles (1, 4 and 10 µm) were used to study particle uptake and transport. A 28-days in vivo feeding study was conducted to analyze transport at the intestinal epithelium and oxidative stress response as a potential consequence of microplastic exposure. Male reporter gene mice were treated three times per week by oral gavage with a mixture of 1 µm (4.55 × 107 particles), 4 µm (4.55 × 107 particles) and 10 µm (1.49 × 106 particles) microplastics at a volume of 10 mL/kg/bw. Effects of particles on macrophage polarization were investigated using the human cell line THP-1 to detect a possible impact on intestinal immune cells. Altogether, the results of the study demonstrate the cellular uptake of a minor fraction of particles. In vivo data show the absence of histologically detectable lesions and inflammatory responses. The particles did not interfere with the differentiation and activation of the human macrophage model. The present results suggest that oral exposure to PS microplastic particles under the chosen experimental conditions does not pose relevant acute health risks to mammals.

Original languageEnglish
Pages (from-to)1817-1833
Number of pages17
JournalArchives of Toxicology
Volume93
Issue number7
Early online date28 May 2019
DOIs
Publication statusPublished - Jul 2019

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Macrophages
Polystyrenes
Mammals
Oxidative stress
Health risks
Nutrition
Genes
Chemical activation
Cells
Health
Polarization
Plastics
Cell Line
Goblet Cells
Macrophage Activation
Intestinal Mucosa
Coculture Techniques
Reporter Genes
Rodentia
Oxidative Stress

Keywords

  • Gastrointestinal barrier
  • HOTT mice
  • Microplastic
  • Oral uptake
  • Particle size

Cite this

Stock, V., Böhmert, L., Lisicki, E., Block, R., Cara-Carmona, J., Pack, L. K., ... Lampen, A. (2019). Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo. Archives of Toxicology, 93(7), 1817-1833. https://doi.org/10.1007/s00204-019-02478-7
Stock, Valerie ; Böhmert, Linda ; Lisicki, Elisa ; Block, Rafael ; Cara-Carmona, Julia ; Pack, Laura Kim ; Selb, Regina ; Lichtenstein, Dajana ; Voss, Linn ; Henderson, Colin J. ; Zabinsky, Elke ; Sieg, Holger ; Braeuning, Albert ; Lampen, Alfonso. / Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo. In: Archives of Toxicology. 2019 ; Vol. 93, No. 7. pp. 1817-1833.
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title = "Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo",
abstract = "Evidence exists that humans are exposed to plastic microparticles via diet. Data on intestinal particle uptake and health-related effects resulting from microplastic exposure are scarce. Aim of the study was to analyze the uptake and effects of microplastic particles in human in vitro systems and in rodents in vivo. The gastrointestinal uptake of microplastics was studied in vitro using the human intestinal epithelial cell line Caco-2 and thereof-derived co-cultures mimicking intestinal M-cells and goblet cells. Different sizes of spherical fluorescent polystyrene (PS) particles (1, 4 and 10 µm) were used to study particle uptake and transport. A 28-days in vivo feeding study was conducted to analyze transport at the intestinal epithelium and oxidative stress response as a potential consequence of microplastic exposure. Male reporter gene mice were treated three times per week by oral gavage with a mixture of 1 µm (4.55 × 107 particles), 4 µm (4.55 × 107 particles) and 10 µm (1.49 × 106 particles) microplastics at a volume of 10 mL/kg/bw. Effects of particles on macrophage polarization were investigated using the human cell line THP-1 to detect a possible impact on intestinal immune cells. Altogether, the results of the study demonstrate the cellular uptake of a minor fraction of particles. In vivo data show the absence of histologically detectable lesions and inflammatory responses. The particles did not interfere with the differentiation and activation of the human macrophage model. The present results suggest that oral exposure to PS microplastic particles under the chosen experimental conditions does not pose relevant acute health risks to mammals.",
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author = "Valerie Stock and Linda B{\"o}hmert and Elisa Lisicki and Rafael Block and Julia Cara-Carmona and Pack, {Laura Kim} and Regina Selb and Dajana Lichtenstein and Linn Voss and Henderson, {Colin J.} and Elke Zabinsky and Holger Sieg and Albert Braeuning and Alfonso Lampen",
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Stock, V, Böhmert, L, Lisicki, E, Block, R, Cara-Carmona, J, Pack, LK, Selb, R, Lichtenstein, D, Voss, L, Henderson, CJ, Zabinsky, E, Sieg, H, Braeuning, A & Lampen, A 2019, 'Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo', Archives of Toxicology, vol. 93, no. 7, pp. 1817-1833. https://doi.org/10.1007/s00204-019-02478-7

Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo. / Stock, Valerie; Böhmert, Linda (Lead / Corresponding author); Lisicki, Elisa; Block, Rafael; Cara-Carmona, Julia; Pack, Laura Kim; Selb, Regina; Lichtenstein, Dajana; Voss, Linn; Henderson, Colin J.; Zabinsky, Elke; Sieg, Holger; Braeuning, Albert; Lampen, Alfonso.

In: Archives of Toxicology, Vol. 93, No. 7, 07.2019, p. 1817-1833.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Uptake and effects of orally ingested polystyrene microplastic particles in vitro and in vivo

AU - Stock, Valerie

AU - Böhmert, Linda

AU - Lisicki, Elisa

AU - Block, Rafael

AU - Cara-Carmona, Julia

AU - Pack, Laura Kim

AU - Selb, Regina

AU - Lichtenstein, Dajana

AU - Voss, Linn

AU - Henderson, Colin J.

AU - Zabinsky, Elke

AU - Sieg, Holger

AU - Braeuning, Albert

AU - Lampen, Alfonso

N1 - This work was supported by the German Federal Institute for Risk Assessment (Projects 1322-675, 1322-622 and 1323-102).

PY - 2019/7

Y1 - 2019/7

N2 - Evidence exists that humans are exposed to plastic microparticles via diet. Data on intestinal particle uptake and health-related effects resulting from microplastic exposure are scarce. Aim of the study was to analyze the uptake and effects of microplastic particles in human in vitro systems and in rodents in vivo. The gastrointestinal uptake of microplastics was studied in vitro using the human intestinal epithelial cell line Caco-2 and thereof-derived co-cultures mimicking intestinal M-cells and goblet cells. Different sizes of spherical fluorescent polystyrene (PS) particles (1, 4 and 10 µm) were used to study particle uptake and transport. A 28-days in vivo feeding study was conducted to analyze transport at the intestinal epithelium and oxidative stress response as a potential consequence of microplastic exposure. Male reporter gene mice were treated three times per week by oral gavage with a mixture of 1 µm (4.55 × 107 particles), 4 µm (4.55 × 107 particles) and 10 µm (1.49 × 106 particles) microplastics at a volume of 10 mL/kg/bw. Effects of particles on macrophage polarization were investigated using the human cell line THP-1 to detect a possible impact on intestinal immune cells. Altogether, the results of the study demonstrate the cellular uptake of a minor fraction of particles. In vivo data show the absence of histologically detectable lesions and inflammatory responses. The particles did not interfere with the differentiation and activation of the human macrophage model. The present results suggest that oral exposure to PS microplastic particles under the chosen experimental conditions does not pose relevant acute health risks to mammals.

AB - Evidence exists that humans are exposed to plastic microparticles via diet. Data on intestinal particle uptake and health-related effects resulting from microplastic exposure are scarce. Aim of the study was to analyze the uptake and effects of microplastic particles in human in vitro systems and in rodents in vivo. The gastrointestinal uptake of microplastics was studied in vitro using the human intestinal epithelial cell line Caco-2 and thereof-derived co-cultures mimicking intestinal M-cells and goblet cells. Different sizes of spherical fluorescent polystyrene (PS) particles (1, 4 and 10 µm) were used to study particle uptake and transport. A 28-days in vivo feeding study was conducted to analyze transport at the intestinal epithelium and oxidative stress response as a potential consequence of microplastic exposure. Male reporter gene mice were treated three times per week by oral gavage with a mixture of 1 µm (4.55 × 107 particles), 4 µm (4.55 × 107 particles) and 10 µm (1.49 × 106 particles) microplastics at a volume of 10 mL/kg/bw. Effects of particles on macrophage polarization were investigated using the human cell line THP-1 to detect a possible impact on intestinal immune cells. Altogether, the results of the study demonstrate the cellular uptake of a minor fraction of particles. In vivo data show the absence of histologically detectable lesions and inflammatory responses. The particles did not interfere with the differentiation and activation of the human macrophage model. The present results suggest that oral exposure to PS microplastic particles under the chosen experimental conditions does not pose relevant acute health risks to mammals.

KW - Gastrointestinal barrier

KW - HOTT mice

KW - Microplastic

KW - Oral uptake

KW - Particle size

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EP - 1833

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