Uptake of cobalt and cesium by microalgal- and cyanobacterial-clay mixtures

Accumulation of cobalt and cesium by the microalga Scenedesmus obliquus and the cyanobacterium Synechocystis PCC 6803 has been characterized at metal concentrations ranging from 1-100 μM in the presence of three clay minerals, montmorillonite, illite, and kaolinite. The majority of metal uptake over a 4 h period consisted of rapid binding to the clay mineral-cell aggregates, and was unaffected by incubation in the dark or by the presence of the metabolic inhibitor carbonyl cyanide-3-chlorophenyl hydrazone (CCCP). This was followed by a slower, energy-dependent uptake of metal by the cell components of the mixtures, which was inhibited by incubation in the dark or in the presence of CCCP. The initial phase of uptake by the clay mineral-cell mixtures and mixture components alone conformed to a Freundlich adsorption isotherm, the order of uptake for both cobalt and cesium being montmorillonite-cells > illite-cells > kaolinite-cells. S. obliquus-clay mineral mixtures accumulated more cobalt and cesium than Synechocystis PCC 6803-clay mineral mixtures. On a dry weight basis, clay minerals alone accumulated greater amounts of metals than clay mineral-cell mixtures, which accumulated more than the cells alone. However, when the same data was expressed as amount of metal adsorbed per unit surface area, S. obliquus, in most cases, adsorbed greater amounts of cobalt and cesium than the clay minerals or Synechocystis PCC 6803. As the proportion of clay in a cell-clay mineral mixture was increased, the amount of metal accumulated also increased. Reduced accumulation of cobalt and cesium by cell-clay mineral mixtures, exhibited by equal amounts of the individual components added together, indicated that the formation of clay-cell aggregates had masked some of the binding sites normally available to metal ions. Accumulation of cobalt and cesium by all clay mineral-cell mixtures was dependent on the external pH and NaCl concentration, and decreased with decreasing pH and increasing external NaCl concentration.