Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

Xinjin Liang; Laszlo Csetenyi; Geoffrey Michael Gadd

doi:10.1007/s00253-016-7327-9

Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

Xinjin Liang, Laszlo Csetenyi, Geoffrey Michael Gadd (Lead / Corresponding author)

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Abstract

In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C₃H₇Na₂O₆P·xH₂O (G2P)) or phytic acid sodium salt hydrate (C₆H₁₈O₂₄P₆·xNa⁺·yH₂O (PyA))) in the presence of soluble UO₂(NO₃)₂. The formation of meta-ankoleite (K₂(UO₂)₂(PO₄)₂·6(H₂O)), chernikovite ((H₃O)₂(UO₂)₂(PO₄)₂·6(H₂O)), bassetite (Fe⁺⁺(UO₂)₂(PO₄)₂·8(H₂O)), and uramphite ((NH₄)(UO₂)(PO₄)·3(H₂O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO₂(NO₃)₂. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.

Original language	English
Pages (from-to)	5141-5151
Number of pages	11
Journal	Applied Microbiology and Biotechnology
Volume	100
Issue number	11
Early online date	5 Feb 2016
DOIs	https://doi.org/10.1007/s00253-016-7327-9
Publication status	Published - Jun 2016

Keywords

Biomineralization
Bioprecipitation
Fungi
Glycerol 2-phosphate
Phytic acid
Uranium
Yeasts

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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The final publication is available at Springer via http://dx.doi.org/10.1007/s00253-016-7327-9
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Cite this

@article{c17642702090477393169a5e8bc61b51,

title = "Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates",

abstract = "In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.",

keywords = "Biomineralization, Bioprecipitation, Fungi, Glycerol 2-phosphate, Phytic acid, Uranium, Yeasts",

author = "Xinjin Liang and Laszlo Csetenyi and Gadd, {Geoffrey Michael}",

note = "G. M. Gadd also gratefully acknowledges an award under the Chinese Government{\textquoteright}s 1000 Talents Plan with the Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.",

year = "2016",

month = jun,

doi = "10.1007/s00253-016-7327-9",

language = "English",

volume = "100",

pages = "5141--5151",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

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T1 - Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

AU - Liang, Xinjin

AU - Csetenyi, Laszlo

AU - Gadd, Geoffrey Michael

N1 - G. M. Gadd also gratefully acknowledges an award under the Chinese Government’s 1000 Talents Plan with the Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.

PY - 2016/6

Y1 - 2016/6

N2 - In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.

AB - In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.

KW - Biomineralization

KW - Bioprecipitation

KW - Fungi

KW - Glycerol 2-phosphate

KW - Phytic acid

KW - Uranium

KW - Yeasts

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U2 - 10.1007/s00253-016-7327-9

DO - 10.1007/s00253-016-7327-9

M3 - Article

C2 - 26846744

AN - SCOPUS:84957598813

SN - 0175-7598

VL - 100

SP - 5141

EP - 5151

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 11

ER -

Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

Abstract

Keywords

ASJC Scopus subject areas

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Tellurium and Selenium Cycling and Supply (Joint with Universities of Leicester, Durham, Edinburgh, Cardiff, Aberdeen and Open University and Natural History Museum)

COG3: The Geology, Geometallurgy and Geomicrobiology of Cobalt Resources Leading to New Product Streams (joint with Natural History Museum and Universities of Manchester, Bangor, Exeter, Loughborough and Southampton and Industrial Partner)

Cite this

Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Tellurium and Selenium Cycling and Supply (Joint with Universities of Leicester, Durham, Edinburgh, Cardiff, Aberdeen and Open University and Natural History Museum)

COG3: The Geology, Geometallurgy and Geomicrobiology of Cobalt Resources Leading to New Product Streams (joint with Natural History Museum and Universities of Manchester, Bangor, Exeter, Loughborough and Southampton and Industrial Partner)

Cite this