Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

Xinjin Liang, Laszlo Csetenyi, Geoffrey Michael Gadd (Lead / Corresponding author)

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Abstract

In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.

Original languageEnglish
Pages (from-to)5141-5151
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume100
Issue number11
Early online date5 Feb 2016
DOIs
Publication statusPublished - Jun 2016

Fingerprint

Uranium
Phosphorus
Yeasts
Organophosphates
Salts
Phosphates
Phytic Acid
Phosphoric Monoester Hydrolases
X-Ray Diffraction
Glycerol
Minerals
Sodium
Research

Keywords

  • Biomineralization
  • Bioprecipitation
  • Fungi
  • Glycerol 2-phosphate
  • Phytic acid
  • Uranium
  • Yeasts

Cite this

@article{c17642702090477393169a5e8bc61b51,
title = "Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates",
abstract = "In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.",
keywords = "Biomineralization, Bioprecipitation, Fungi, Glycerol 2-phosphate, Phytic acid, Uranium, Yeasts",
author = "Xinjin Liang and Laszlo Csetenyi and Gadd, {Geoffrey Michael}",
note = "G. M. Gadd also gratefully acknowledges an award under the Chinese Government’s 1000 Talents Plan with the Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.",
year = "2016",
month = "6",
doi = "10.1007/s00253-016-7327-9",
language = "English",
volume = "100",
pages = "5141--5151",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
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TY - JOUR

T1 - Uranium bioprecipitation mediated by yeasts utilizing organic phosphorus substrates

AU - Liang, Xinjin

AU - Csetenyi, Laszlo

AU - Gadd, Geoffrey Michael

N1 - G. M. Gadd also gratefully acknowledges an award under the Chinese Government’s 1000 Talents Plan with the Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, China.

PY - 2016/6

Y1 - 2016/6

N2 - In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.

AB - In this research, we have demonstrated the ability of several yeast species to mediate U(VI) biomineralization through uranium phosphate biomineral formation when utilizing an organic source of phosphorus (glycerol 2-phosphate disodium salt hydrate (C3H7Na2O6P·xH2O (G2P)) or phytic acid sodium salt hydrate (C6H18O24P6·xNa+·yH2O (PyA))) in the presence of soluble UO2(NO3)2. The formation of meta-ankoleite (K2(UO2)2(PO4)2·6(H2O)), chernikovite ((H3O)2(UO2)2(PO4)2·6(H2O)), bassetite (Fe++(UO2)2(PO4)2·8(H2O)), and uramphite ((NH4)(UO2)(PO4)·3(H2O)) on cell surfaces was confirmed by X-ray diffraction in yeasts grown in a defined liquid medium amended with uranium and an organic phosphorus source, as well as in yeasts pre-grown in organic phosphorus-containing media and then subsequently exposed to UO2(NO3)2. The resulting minerals depended on the yeast species as well as physico-chemical conditions. The results obtained in this study demonstrate that phosphatase-mediated uranium biomineralization can occur in yeasts supplied with an organic phosphate substrate as sole source of phosphorus. Further understanding of yeast interactions with uranium may be relevant to development of potential treatment methods for uranium waste and utilization of organic phosphate sources and for prediction of microbial impacts on the fate of uranium in the environment.

KW - Biomineralization

KW - Bioprecipitation

KW - Fungi

KW - Glycerol 2-phosphate

KW - Phytic acid

KW - Uranium

KW - Yeasts

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U2 - 10.1007/s00253-016-7327-9

DO - 10.1007/s00253-016-7327-9

M3 - Article

VL - 100

SP - 5141

EP - 5151

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 11

ER -