Uranium phosphate biomineralization by fungi

Xinjin Liang, Stephen Hillier, Helen Pendlowski, Nia Gray, Andrea Ceci, Geoffrey Michael Gadd (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Geoactive soil fungi were investigated for phosphatase-mediated uranium precipitation during growth on an organic phosphorus source. Aspergillus niger and Paecilomyces javanicus were grown on modified Czapek-Dox medium amended with glycerol 2-phosphate (G2P) as sole P source and uranium nitrate. Both organisms showed reduced growth on uranium-containing media but were able to extensively precipitate uranium and phosphorus-containing minerals on hyphal surfaces, and these were identified by X-ray powder diffraction as uranyl phosphate species, including potassium uranyl phosphate hydrate (KPUO6.3H2O), meta-ankoleite [(K1.7Ba0.2)(UO2)2(PO4)2.6H2O], uranyl phosphate hydrate [(UO2)3(PO4)2.4H2O], meta-ankoleite (K(UO2)(PO4).3H2O), uramphite (NH4UO2PO4.3H2O) and chernikovite [(H3O)2(UO2)2(PO4)2.6H2O]. Some minerals with a morphology similar to bacterial hydrogen uranyl phosphate were detected on A. niger biomass. Geochemical modelling confirmed the complexity of uranium speciation, and the presence of meta-ankoleite, uramphite and uranyl phosphate hydrate between pH 3 and 8 closely matched the experimental data, with potassium as the dominant cation. We have therefore demonstrated that fungi can precipitate U-containing phosphate biominerals when grown with an organic source of P, with the hyphal matrix serving to localize the resultant uranium minerals. The findings throw further light on potential fungal roles in U and P biogeochemistry as well as the application of these mechanisms for element recovery or bioremediation.

    Original languageEnglish
    Pages (from-to)2064-2075
    Number of pages12
    JournalEnvironmental Microbiology
    Volume17
    Issue number6
    Early online date10 Mar 2015
    DOIs
    Publication statusPublished - Jun 2015

    Fingerprint

    biomineralization
    Uranium
    uranium
    Fungi
    Phosphates
    phosphate
    fungus
    phosphates
    fungi
    Minerals
    Aspergillus niger
    Phosphorus
    minerals
    Uranyl Nitrate
    Paecilomyces
    Powder Diffraction
    Environmental Biodegradation
    mineral
    potassium
    phosphorus

    Cite this

    Liang, X., Hillier, S., Pendlowski, H., Gray, N., Ceci, A., & Gadd, G. M. (2015). Uranium phosphate biomineralization by fungi. Environmental Microbiology, 17(6), 2064-2075. https://doi.org/10.1111/1462-2920.12771
    Liang, Xinjin ; Hillier, Stephen ; Pendlowski, Helen ; Gray, Nia ; Ceci, Andrea ; Gadd, Geoffrey Michael. / Uranium phosphate biomineralization by fungi. In: Environmental Microbiology. 2015 ; Vol. 17, No. 6. pp. 2064-2075.
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    abstract = "Geoactive soil fungi were investigated for phosphatase-mediated uranium precipitation during growth on an organic phosphorus source. Aspergillus niger and Paecilomyces javanicus were grown on modified Czapek-Dox medium amended with glycerol 2-phosphate (G2P) as sole P source and uranium nitrate. Both organisms showed reduced growth on uranium-containing media but were able to extensively precipitate uranium and phosphorus-containing minerals on hyphal surfaces, and these were identified by X-ray powder diffraction as uranyl phosphate species, including potassium uranyl phosphate hydrate (KPUO6.3H2O), meta-ankoleite [(K1.7Ba0.2)(UO2)2(PO4)2.6H2O], uranyl phosphate hydrate [(UO2)3(PO4)2.4H2O], meta-ankoleite (K(UO2)(PO4).3H2O), uramphite (NH4UO2PO4.3H2O) and chernikovite [(H3O)2(UO2)2(PO4)2.6H2O]. Some minerals with a morphology similar to bacterial hydrogen uranyl phosphate were detected on A. niger biomass. Geochemical modelling confirmed the complexity of uranium speciation, and the presence of meta-ankoleite, uramphite and uranyl phosphate hydrate between pH 3 and 8 closely matched the experimental data, with potassium as the dominant cation. We have therefore demonstrated that fungi can precipitate U-containing phosphate biominerals when grown with an organic source of P, with the hyphal matrix serving to localize the resultant uranium minerals. The findings throw further light on potential fungal roles in U and P biogeochemistry as well as the application of these mechanisms for element recovery or bioremediation.",
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    Liang, X, Hillier, S, Pendlowski, H, Gray, N, Ceci, A & Gadd, GM 2015, 'Uranium phosphate biomineralization by fungi', Environmental Microbiology, vol. 17, no. 6, pp. 2064-2075. https://doi.org/10.1111/1462-2920.12771

    Uranium phosphate biomineralization by fungi. / Liang, Xinjin; Hillier, Stephen; Pendlowski, Helen; Gray, Nia; Ceci, Andrea; Gadd, Geoffrey Michael (Lead / Corresponding author).

    In: Environmental Microbiology, Vol. 17, No. 6, 06.2015, p. 2064-2075.

    Research output: Contribution to journalArticle

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    AB - Geoactive soil fungi were investigated for phosphatase-mediated uranium precipitation during growth on an organic phosphorus source. Aspergillus niger and Paecilomyces javanicus were grown on modified Czapek-Dox medium amended with glycerol 2-phosphate (G2P) as sole P source and uranium nitrate. Both organisms showed reduced growth on uranium-containing media but were able to extensively precipitate uranium and phosphorus-containing minerals on hyphal surfaces, and these were identified by X-ray powder diffraction as uranyl phosphate species, including potassium uranyl phosphate hydrate (KPUO6.3H2O), meta-ankoleite [(K1.7Ba0.2)(UO2)2(PO4)2.6H2O], uranyl phosphate hydrate [(UO2)3(PO4)2.4H2O], meta-ankoleite (K(UO2)(PO4).3H2O), uramphite (NH4UO2PO4.3H2O) and chernikovite [(H3O)2(UO2)2(PO4)2.6H2O]. Some minerals with a morphology similar to bacterial hydrogen uranyl phosphate were detected on A. niger biomass. Geochemical modelling confirmed the complexity of uranium speciation, and the presence of meta-ankoleite, uramphite and uranyl phosphate hydrate between pH 3 and 8 closely matched the experimental data, with potassium as the dominant cation. We have therefore demonstrated that fungi can precipitate U-containing phosphate biominerals when grown with an organic source of P, with the hyphal matrix serving to localize the resultant uranium minerals. The findings throw further light on potential fungal roles in U and P biogeochemistry as well as the application of these mechanisms for element recovery or bioremediation.

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    Liang X, Hillier S, Pendlowski H, Gray N, Ceci A, Gadd GM. Uranium phosphate biomineralization by fungi. Environmental Microbiology. 2015 Jun;17(6):2064-2075. https://doi.org/10.1111/1462-2920.12771