Urban greenspace types influence the microbial community assembly and antibiotic resistome more in the phyllosphere than in the soil

TY - JOUR

T1 - Urban greenspace types influence the microbial community assembly and antibiotic resistome more in the phyllosphere than in the soil

AU - Huang, Xin-Rong

AU - Neilson, Roy

AU - Yang, Le-Yang

AU - Deng, Jing-Jun

AU - Zhou, Shu-Yi-Dan

AU - Li, Hu

AU - Zhu, Yong-Guan

AU - Yang, Xiao-Ru

N1 - Copyright: © 2023 Elsevier Ltd. All rights reserved.

PY - 2023/10

Y1 - 2023/10

N2 - Urban greenspace (UGS) is recognized to confer significant societal benefits, but few studies explored the microbial communities and antibiotic resistance genes (ARGs) from different urban greenspace types. Here, we collected leaf and soil samples from forest, greenbelt, and parkland to analyze microbial community assembly and ARG profile. For phyllosphere fungal community, the α-diversity was higher in forest, compared to those in greenbelt and parkland. Moreover, urban greenspace types altered the community assembly. Stochastic processes had a greater effect on phyllosphere fungal community in greenbelt and parkland, while in forest they were dominated by deterministic processes. In contrast, no significant differences in bacterial community diversity, community assembly were observed between the samples collected from different urban greenspace types. A total of 153 ARGs and mobile genetic elements (MGEs) were detected in phyllosphere and soil with resistance to the majority classes of antibiotics commonly applied to humans and animals. Structural equation model further revealed that a direct association between greenspace type and ARGs in the phyllosphere even after considering the effects of all other factors simultaneously. Our findings provide new insights into the microbial communities and antibiotic resistome of urban greenspaces and the potential risk linked with human health.

AB - Urban greenspace (UGS) is recognized to confer significant societal benefits, but few studies explored the microbial communities and antibiotic resistance genes (ARGs) from different urban greenspace types. Here, we collected leaf and soil samples from forest, greenbelt, and parkland to analyze microbial community assembly and ARG profile. For phyllosphere fungal community, the α-diversity was higher in forest, compared to those in greenbelt and parkland. Moreover, urban greenspace types altered the community assembly. Stochastic processes had a greater effect on phyllosphere fungal community in greenbelt and parkland, while in forest they were dominated by deterministic processes. In contrast, no significant differences in bacterial community diversity, community assembly were observed between the samples collected from different urban greenspace types. A total of 153 ARGs and mobile genetic elements (MGEs) were detected in phyllosphere and soil with resistance to the majority classes of antibiotics commonly applied to humans and animals. Structural equation model further revealed that a direct association between greenspace type and ARGs in the phyllosphere even after considering the effects of all other factors simultaneously. Our findings provide new insights into the microbial communities and antibiotic resistome of urban greenspaces and the potential risk linked with human health.

KW - Microbiota

KW - Antibiotic resistance genes

KW - Potential pathogens

KW - Management practices

KW - Public health

UR - https://www.scopus.com/pages/publications/85165155898

U2 - 10.1016/j.chemosphere.2023.139533

DO - 10.1016/j.chemosphere.2023.139533

M3 - Article

C2 - 37459932

SN - 0045-6535

VL - 338

JO - Chemosphere

JF - Chemosphere

M1 - 139533

ER -