Urinary desmosine, a biomarker of elastin degradation is significantly elevated and associated with maximum aortic root size and aortic Z-scores in patients with bicuspid aortic valve

Zaid Iskandar, Jeffrey Huang, Lynn Miller, Calvin Chin, Ify Mordi, Catherine Mcwilliam, David Goudie, Jonathan Berg, Alex Neagoie, Chim Lang, Anna-Maria Choy

Research output: Contribution to journalMeeting abstract

Abstract

Introduction Bicuspid aortic valve (BAV) affects up to 2% of the general population and is associated with a significant risk of aortic dilatation and aortic dissection. Therefore, patients are normally routinely followed up with serial echocardiography. Aortic dilatation involves elastin breakdown as part of extracellular matrix degradation. Desmosine is a specific amino acid cross-link between mature elastin molecules and is released into plasma and urine following elastin degradation. Following on from previous pilot data in the DESMA study on patients with Marfan Syndrome which demonstrated a significant correlation between plasma desmosine and aortic root size, we explored whether this same relationship is seen in patients with other forms of aortopathies, specifically the BAV cohort.Methods Urinary and plasma desmosine were measured in 20 patients with BAV and compared to age-matched healthy controls. Urinary desmosine (uDES) and plasma desmosine (pDES) levels were measured in the same laboratory using liquid chromatography-tandem mass spectrometry (LC-MS).Results The cohort was predominantly male (75 and mean age was 49.3textpm17.3 years old. All the patients had normal LV systolic function and 9 (45 patients had treated hypertension. Mean aortic root size was 35textpm5.8mm. Compared to controls, both uDES (15.9 textpm 4.6 vs 7.2 textpm 2.8 ng/mg creatinine, plt;0.001) and pDES (0.3 textpm 0.1 vs 0.26 textpm 0.075 ng/mL, p=0.01) were significantly elevated in patients with BAV. Urinary desmosine was significantly correlated (Figure 1) with aortic root size (r=0.65, p=0.002), and aortic root z-score (r=0.59, p=0.007), however there was no significant correlation seen between plasma desmosine and aortic root size (r=0.42, p=0.06) or aortic root z-score (r=0.14, p=0.56).Abstract 126 Figure 1 Correlation between uDES (ng/mg creatinine) and aortic root size (mm)Conclusion Urinary and plasma desmosine levels are significantly higher in patients with BAV compared to controls. Urinary desmosine is also significantly associated with aortic root size and z-score, reflecting higher elastin degradation in patients with larger aortic roots. This suggests a potential utility of desmosine as a biomarker in patients with BAV. Larger studies are needed to test this hypothesis.Conflict of Interest None
Original languageEnglish
Article number126
Pages (from-to)A103-A104
Number of pages2
JournalHeart
Volume105
Issue numberSupplement 6
Early online date1 May 2019
DOIs
Publication statusPublished - 26 May 2019

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Desmosine
Elastin
Biomarkers
Bicuspid Aortic Valve
Dilatation
Creatinine
Conflict of Interest
Marfan Syndrome
Tandem Mass Spectrometry

Cite this

@article{2b56cef7500f4727b84623ef033158f6,
title = "Urinary desmosine, a biomarker of elastin degradation is significantly elevated and associated with maximum aortic root size and aortic Z-scores in patients with bicuspid aortic valve",
abstract = "Introduction Bicuspid aortic valve (BAV) affects up to 2{\%} of the general population and is associated with a significant risk of aortic dilatation and aortic dissection. Therefore, patients are normally routinely followed up with serial echocardiography. Aortic dilatation involves elastin breakdown as part of extracellular matrix degradation. Desmosine is a specific amino acid cross-link between mature elastin molecules and is released into plasma and urine following elastin degradation. Following on from previous pilot data in the DESMA study on patients with Marfan Syndrome which demonstrated a significant correlation between plasma desmosine and aortic root size, we explored whether this same relationship is seen in patients with other forms of aortopathies, specifically the BAV cohort.Methods Urinary and plasma desmosine were measured in 20 patients with BAV and compared to age-matched healthy controls. Urinary desmosine (uDES) and plasma desmosine (pDES) levels were measured in the same laboratory using liquid chromatography-tandem mass spectrometry (LC-MS).Results The cohort was predominantly male (75 and mean age was 49.3textpm17.3 years old. All the patients had normal LV systolic function and 9 (45 patients had treated hypertension. Mean aortic root size was 35textpm5.8mm. Compared to controls, both uDES (15.9 textpm 4.6 vs 7.2 textpm 2.8 ng/mg creatinine, plt;0.001) and pDES (0.3 textpm 0.1 vs 0.26 textpm 0.075 ng/mL, p=0.01) were significantly elevated in patients with BAV. Urinary desmosine was significantly correlated (Figure 1) with aortic root size (r=0.65, p=0.002), and aortic root z-score (r=0.59, p=0.007), however there was no significant correlation seen between plasma desmosine and aortic root size (r=0.42, p=0.06) or aortic root z-score (r=0.14, p=0.56).Abstract 126 Figure 1 Correlation between uDES (ng/mg creatinine) and aortic root size (mm)Conclusion Urinary and plasma desmosine levels are significantly higher in patients with BAV compared to controls. Urinary desmosine is also significantly associated with aortic root size and z-score, reflecting higher elastin degradation in patients with larger aortic roots. This suggests a potential utility of desmosine as a biomarker in patients with BAV. Larger studies are needed to test this hypothesis.Conflict of Interest None",
author = "Zaid Iskandar and Jeffrey Huang and Lynn Miller and Calvin Chin and Ify Mordi and Catherine Mcwilliam and David Goudie and Jonathan Berg and Alex Neagoie and Chim Lang and Anna-Maria Choy",
year = "2019",
month = "5",
day = "26",
doi = "10.1136/heartjnl-2019-BCS.123",
language = "English",
volume = "105",
pages = "A103--A104",
journal = "Heart",
issn = "1355-6037",
publisher = "BMJ Publishing Group",
number = "Supplement 6",

}

Urinary desmosine, a biomarker of elastin degradation is significantly elevated and associated with maximum aortic root size and aortic Z-scores in patients with bicuspid aortic valve. / Iskandar, Zaid; Huang, Jeffrey; Miller, Lynn; Chin, Calvin; Mordi, Ify; Mcwilliam, Catherine; Goudie, David; Berg, Jonathan; Neagoie, Alex; Lang, Chim; Choy, Anna-Maria.

In: Heart, Vol. 105, No. Supplement 6, 126, 26.05.2019, p. A103-A104.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Urinary desmosine, a biomarker of elastin degradation is significantly elevated and associated with maximum aortic root size and aortic Z-scores in patients with bicuspid aortic valve

AU - Iskandar, Zaid

AU - Huang, Jeffrey

AU - Miller, Lynn

AU - Chin, Calvin

AU - Mordi, Ify

AU - Mcwilliam, Catherine

AU - Goudie, David

AU - Berg, Jonathan

AU - Neagoie, Alex

AU - Lang, Chim

AU - Choy, Anna-Maria

PY - 2019/5/26

Y1 - 2019/5/26

N2 - Introduction Bicuspid aortic valve (BAV) affects up to 2% of the general population and is associated with a significant risk of aortic dilatation and aortic dissection. Therefore, patients are normally routinely followed up with serial echocardiography. Aortic dilatation involves elastin breakdown as part of extracellular matrix degradation. Desmosine is a specific amino acid cross-link between mature elastin molecules and is released into plasma and urine following elastin degradation. Following on from previous pilot data in the DESMA study on patients with Marfan Syndrome which demonstrated a significant correlation between plasma desmosine and aortic root size, we explored whether this same relationship is seen in patients with other forms of aortopathies, specifically the BAV cohort.Methods Urinary and plasma desmosine were measured in 20 patients with BAV and compared to age-matched healthy controls. Urinary desmosine (uDES) and plasma desmosine (pDES) levels were measured in the same laboratory using liquid chromatography-tandem mass spectrometry (LC-MS).Results The cohort was predominantly male (75 and mean age was 49.3textpm17.3 years old. All the patients had normal LV systolic function and 9 (45 patients had treated hypertension. Mean aortic root size was 35textpm5.8mm. Compared to controls, both uDES (15.9 textpm 4.6 vs 7.2 textpm 2.8 ng/mg creatinine, plt;0.001) and pDES (0.3 textpm 0.1 vs 0.26 textpm 0.075 ng/mL, p=0.01) were significantly elevated in patients with BAV. Urinary desmosine was significantly correlated (Figure 1) with aortic root size (r=0.65, p=0.002), and aortic root z-score (r=0.59, p=0.007), however there was no significant correlation seen between plasma desmosine and aortic root size (r=0.42, p=0.06) or aortic root z-score (r=0.14, p=0.56).Abstract 126 Figure 1 Correlation between uDES (ng/mg creatinine) and aortic root size (mm)Conclusion Urinary and plasma desmosine levels are significantly higher in patients with BAV compared to controls. Urinary desmosine is also significantly associated with aortic root size and z-score, reflecting higher elastin degradation in patients with larger aortic roots. This suggests a potential utility of desmosine as a biomarker in patients with BAV. Larger studies are needed to test this hypothesis.Conflict of Interest None

AB - Introduction Bicuspid aortic valve (BAV) affects up to 2% of the general population and is associated with a significant risk of aortic dilatation and aortic dissection. Therefore, patients are normally routinely followed up with serial echocardiography. Aortic dilatation involves elastin breakdown as part of extracellular matrix degradation. Desmosine is a specific amino acid cross-link between mature elastin molecules and is released into plasma and urine following elastin degradation. Following on from previous pilot data in the DESMA study on patients with Marfan Syndrome which demonstrated a significant correlation between plasma desmosine and aortic root size, we explored whether this same relationship is seen in patients with other forms of aortopathies, specifically the BAV cohort.Methods Urinary and plasma desmosine were measured in 20 patients with BAV and compared to age-matched healthy controls. Urinary desmosine (uDES) and plasma desmosine (pDES) levels were measured in the same laboratory using liquid chromatography-tandem mass spectrometry (LC-MS).Results The cohort was predominantly male (75 and mean age was 49.3textpm17.3 years old. All the patients had normal LV systolic function and 9 (45 patients had treated hypertension. Mean aortic root size was 35textpm5.8mm. Compared to controls, both uDES (15.9 textpm 4.6 vs 7.2 textpm 2.8 ng/mg creatinine, plt;0.001) and pDES (0.3 textpm 0.1 vs 0.26 textpm 0.075 ng/mL, p=0.01) were significantly elevated in patients with BAV. Urinary desmosine was significantly correlated (Figure 1) with aortic root size (r=0.65, p=0.002), and aortic root z-score (r=0.59, p=0.007), however there was no significant correlation seen between plasma desmosine and aortic root size (r=0.42, p=0.06) or aortic root z-score (r=0.14, p=0.56).Abstract 126 Figure 1 Correlation between uDES (ng/mg creatinine) and aortic root size (mm)Conclusion Urinary and plasma desmosine levels are significantly higher in patients with BAV compared to controls. Urinary desmosine is also significantly associated with aortic root size and z-score, reflecting higher elastin degradation in patients with larger aortic roots. This suggests a potential utility of desmosine as a biomarker in patients with BAV. Larger studies are needed to test this hypothesis.Conflict of Interest None

U2 - 10.1136/heartjnl-2019-BCS.123

DO - 10.1136/heartjnl-2019-BCS.123

M3 - Meeting abstract

VL - 105

SP - A103-A104

JO - Heart

JF - Heart

SN - 1355-6037

IS - Supplement 6

M1 - 126

ER -