Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

Jose Carlos Paredes Franco; Lucia Sampaio Guther; Michael Ferguson

doi:10.1093/glycob/cwae097

Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

Jose Carlos Paredes Franco, Lucia Sampaio Guther, Michael Ferguson (Lead / Corresponding author)

Research output: Contribution to journal › Article › peer-review

23 Downloads (Pure)

Abstract

For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.

Original language	English
Article number	cwae097
Number of pages	6
Journal	Glycobiology
Volume	35
Issue number	2
DOIs	https://doi.org/10.1093/glycob/cwae097
Publication status	Published - 21 Dec 2024

Access to Document

10.1093/glycob/cwae097Licence: CC BY

Final Published VersionFinal published version, 566 KBLicence: CC BY

Protein Glycosylation in Trypanosomes: Defining and Exploiting a Biological System (Senior Investigator Award)
Ferguson, M. (Investigator)
1/10/13 → 30/06/23
Project: Research

Cite this

@article{8bfee3324c6a4af09a4e3c3dab7d9792,

title = "Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose",

abstract = "For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.",

author = "{Paredes Franco}, {Jose Carlos} and {Sampaio Guther}, Lucia and Michael Ferguson",

year = "2024",

month = dec,

day = "21",

doi = "10.1093/glycob/cwae097",

language = "English",

volume = "35",

journal = "Glycobiology",

issn = "0959-6658",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

AU - Paredes Franco, Jose Carlos

AU - Sampaio Guther, Lucia

AU - Ferguson, Michael

PY - 2024/12/21

Y1 - 2024/12/21

N2 - For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.

AB - For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.

U2 - 10.1093/glycob/cwae097

DO - 10.1093/glycob/cwae097

M3 - Article

C2 - 39706805

SN - 0959-6658

VL - 35

JO - Glycobiology

JF - Glycobiology

IS - 2

M1 - cwae097

ER -

Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

Abstract

Access to Document

Fingerprint

Projects

Protein Glycosylation in Trypanosomes: Defining and Exploiting a Biological System (Senior Investigator Award)

Cite this