Projects per year
Abstract
For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.
Original language | English |
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Journal | Glycobiology |
DOIs | |
Publication status | Published - 21 Dec 2024 |
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Dive into the research topics of 'Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose'. Together they form a unique fingerprint.Projects
- 1 Finished
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Protein Glycosylation in Trypanosomes: Defining and Exploiting a Biological System (Senior Investigator Award)
Ferguson, M. (Investigator)
1/10/13 → 30/06/23
Project: Research