Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose

Jose Carlos Paredes Franco, Lucia Sampaio Guther, Michael Ferguson (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    1 Downloads (Pure)

    Abstract

    For studies involving glycosyltransferases and nucleotide sugar transporters, radioactive nucleotide sugars are critical reagents. Of these, GDP-L-[3H]Fucose is currently commercially unavailable. Here, we present a facile approach for the preparation of GDP-[3H]-L-Fucose, using the enzymatic machinery present in the cytosol of the non-infectious and easily cultivated protozoan, Crithidia fasciculata, and its purification by solid phase extraction ion exchange chromatography.
    Original languageEnglish
    JournalGlycobiology
    DOIs
    Publication statusPublished - 21 Dec 2024

    Fingerprint

    Dive into the research topics of 'Use of Crithidia fasciculata extract for the facile enzymatic synthesis of GDP-L-[3H]Fucose'. Together they form a unique fingerprint.

    Cite this