Uterine flushing

a method to recover spermatozoa and leukocytes

M. Williams, L. A. Thompson, T. C. Li, A. Mackenna, C L Barratt, I. D. Cooke

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The process of sperm transport from the cervix, where a leukocytic reaction is initiated, through the uterus to gain access to the site of fertilization is very poorly understood. This preliminary study was designed to utilize a uterine flushing technique to determine firstly, the number of spermatozoa that can be recovered from the uterine cavity at 4 h post-insemination, around the time of ovulation, and secondly, to establish whether the spermatozoa initiate a leukocytic response while present. Uterine flushing was carried out in 10 potentially fertile women at 4 h post-insemination with donor semen, 24-36 h after the onset of the luteinizing hormone (LH) surge. The flush fluid was analysed for the numbers of spermatozoa and leukocytes present. In 8/10 women spermatozoa were retrieved from the uterus, in consistently low numbers (median 46, range 3-415). In 5/5 women leukocytes were recovered (median, 2.75 x 10(8)/l, range 2.0 x 10(8)-12.7 x 10(8)/l) from an origin other than peripheral blood contamination. These results suggest firstly that the flushing technique was a consistent method for retrieving spermatozoa and leukocytes from the uterine cavity, secondly that only low numbers of spermatozoa can be retrieved on flushing and thirdly that the leukocytic reaction to spermatozoa extends to the uterine cavity.

Original languageEnglish
Pages (from-to)925-928
Number of pages4
JournalHuman Reproduction
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 1993

Fingerprint

Spermatozoa
Leukocytes
Insemination
Uterus
Sperm Transport
Luteinizing Hormone
Ovulation
Leukocyte Count
Fertilization
Cervix Uteri
Tissue Donors

Keywords

  • Female
  • Humans
  • Leukocyte Count
  • Leukocytes/immunology
  • Male
  • Sperm Count
  • Sperm Transport/physiology
  • Spermatozoa/immunology
  • Therapeutic Irrigation/methods
  • Uterus

Cite this

Williams, M. ; Thompson, L. A. ; Li, T. C. ; Mackenna, A. ; Barratt, C L ; Cooke, I. D. / Uterine flushing : a method to recover spermatozoa and leukocytes. In: Human Reproduction. 1993 ; Vol. 8, No. 6. pp. 925-928.
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Williams, M, Thompson, LA, Li, TC, Mackenna, A, Barratt, CL & Cooke, ID 1993, 'Uterine flushing: a method to recover spermatozoa and leukocytes', Human Reproduction, vol. 8, no. 6, pp. 925-928. https://doi.org/10.1093/oxfordjournals.humrep.a138168

Uterine flushing : a method to recover spermatozoa and leukocytes. / Williams, M.; Thompson, L. A.; Li, T. C.; Mackenna, A.; Barratt, C L; Cooke, I. D.

In: Human Reproduction, Vol. 8, No. 6, 06.1993, p. 925-928.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Uterine flushing

T2 - a method to recover spermatozoa and leukocytes

AU - Williams, M.

AU - Thompson, L. A.

AU - Li, T. C.

AU - Mackenna, A.

AU - Barratt, C L

AU - Cooke, I. D.

PY - 1993/6

Y1 - 1993/6

N2 - The process of sperm transport from the cervix, where a leukocytic reaction is initiated, through the uterus to gain access to the site of fertilization is very poorly understood. This preliminary study was designed to utilize a uterine flushing technique to determine firstly, the number of spermatozoa that can be recovered from the uterine cavity at 4 h post-insemination, around the time of ovulation, and secondly, to establish whether the spermatozoa initiate a leukocytic response while present. Uterine flushing was carried out in 10 potentially fertile women at 4 h post-insemination with donor semen, 24-36 h after the onset of the luteinizing hormone (LH) surge. The flush fluid was analysed for the numbers of spermatozoa and leukocytes present. In 8/10 women spermatozoa were retrieved from the uterus, in consistently low numbers (median 46, range 3-415). In 5/5 women leukocytes were recovered (median, 2.75 x 10(8)/l, range 2.0 x 10(8)-12.7 x 10(8)/l) from an origin other than peripheral blood contamination. These results suggest firstly that the flushing technique was a consistent method for retrieving spermatozoa and leukocytes from the uterine cavity, secondly that only low numbers of spermatozoa can be retrieved on flushing and thirdly that the leukocytic reaction to spermatozoa extends to the uterine cavity.

AB - The process of sperm transport from the cervix, where a leukocytic reaction is initiated, through the uterus to gain access to the site of fertilization is very poorly understood. This preliminary study was designed to utilize a uterine flushing technique to determine firstly, the number of spermatozoa that can be recovered from the uterine cavity at 4 h post-insemination, around the time of ovulation, and secondly, to establish whether the spermatozoa initiate a leukocytic response while present. Uterine flushing was carried out in 10 potentially fertile women at 4 h post-insemination with donor semen, 24-36 h after the onset of the luteinizing hormone (LH) surge. The flush fluid was analysed for the numbers of spermatozoa and leukocytes present. In 8/10 women spermatozoa were retrieved from the uterus, in consistently low numbers (median 46, range 3-415). In 5/5 women leukocytes were recovered (median, 2.75 x 10(8)/l, range 2.0 x 10(8)-12.7 x 10(8)/l) from an origin other than peripheral blood contamination. These results suggest firstly that the flushing technique was a consistent method for retrieving spermatozoa and leukocytes from the uterine cavity, secondly that only low numbers of spermatozoa can be retrieved on flushing and thirdly that the leukocytic reaction to spermatozoa extends to the uterine cavity.

KW - Female

KW - Humans

KW - Leukocyte Count

KW - Leukocytes/immunology

KW - Male

KW - Sperm Count

KW - Sperm Transport/physiology

KW - Spermatozoa/immunology

KW - Therapeutic Irrigation/methods

KW - Uterus

U2 - 10.1093/oxfordjournals.humrep.a138168

DO - 10.1093/oxfordjournals.humrep.a138168

M3 - Article

VL - 8

SP - 925

EP - 928

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 6

ER -