Utilizing thermal proteome profiling to identify the molecular targets of anti-leishmanial compounds

Victoriano Corpas Lopez (Lead / Corresponding author), Susan Wyllie (Lead / Corresponding author)

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7 Citations (Scopus)
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Abstract

Here we detail our optimized protocol for the identification of drug targets in Leishmania donovani using thermal proteome profiling (TPP). This approach is based on the principle that binding of a drug to its protein target can significantly alter the thermal stability of that protein. By monitoring changes in the thermal stability of proteins within drug-treated and untreated cell lysates, using mass spectrometry combined with tandem-mass-tag labelling, putative targets of the drug can be identified in an unbiased manner.
Original languageEnglish
Article number100704
Number of pages19
JournalSTAR Protocols
Volume2
Issue number3
Early online date18 Aug 2021
DOIs
Publication statusPublished - 17 Sept 2021

Keywords

  • Biophysics
  • Microbiology
  • Protein Biochemistry
  • Proteomics
  • Mass Spectrometry

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