Projects per year
Abstract
Here we detail our optimized protocol for the identification of drug targets in Leishmania donovani using thermal proteome profiling (TPP). This approach is based on the principle that binding of a drug to its protein target can significantly alter the thermal stability of that protein. By monitoring changes in the thermal stability of proteins within drug-treated and untreated cell lysates, using mass spectrometry combined with tandem-mass-tag labelling, putative targets of the drug can be identified in an unbiased manner.
Original language | English |
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Article number | 100704 |
Number of pages | 19 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 3 |
Early online date | 18 Aug 2021 |
DOIs | |
Publication status | Published - 17 Sept 2021 |
Keywords
- Biophysics
- Microbiology
- Protein Biochemistry
- Proteomics
- Mass Spectrometry
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A Platform for Drug Target Deconvolution and Exploitation
Gilbert, I., Horn, D., Pawlowic, M., Wyatt, P. & Wyllie, S.
31/12/20 → 30/12/23
Project: Research
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Wellcome Centre for Anti-Infectives Research
Cook, S., De Rycker, M., Fairlamb, A., Ferguson, M., Field, M., Gilbert, I., Gray, D., Horn, D., Pawlowic, M., Read, K., Wyatt, P. & Wyllie, S.
1/04/17 → 31/03/24
Project: Research
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Chemical Biology: Leveraging Phenotypic Hits Against Kinetoplastids (Strategic Grant)
Fairlamb, A., Field, M., Gilbert, I., Gray, D., Horn, D. & Wyatt, P.
1/01/15 → 31/12/20
Project: Research
Equipment
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Fingerprints Proteomics Facility
Centre for Advanced Scientific TechnologiesFacility/equipment: Facility