UV-induced fragmentation of Cajal bodies

Mario Cioce, Severine Boulon, A. Gregory Matera, Angus I. Lamond

    Research output: Contribution to journalArticlepeer-review

    79 Citations (Scopus)
    465 Downloads (Pure)

    Abstract

    The morphology and composition of subnuclear organelles, such as Cajal bodies (CBs), nucleoli, and other nuclear bodies, is dynamic and can change in response to a variety of cell stimuli, including stress. We show that UV-C irradiation disrupts CBs and alters the distribution of a specific subset of CB components. The effect of UV-C on CBs differs from previously reported effects of transcription inhibitors. We demonstrate that the mechanism underlying the response of CBs to UV-C is mediated, at least in part, by PA28? (proteasome activator subunit ?). The presence of PA28? in coilin-containing complexes is increased by UV-C. Overexpression of PA28?, in the absence of UV-C treatment, provokes a similar redistribution of the same subset of CB components that respond to UV-C. RNA interference–mediated knockdown of PA28? attenuates the nuclear disruption caused by UV-C. These data demonstrate that CBs are specific nuclear targets of cellular stress-response pathways and identify PA28? as a novel regulator of CB integrity.

    © 2006 Cioce et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

    Original languageEnglish
    Pages (from-to)401-413
    Number of pages13
    JournalJournal of Cell Biology
    Volume175
    Issue number3
    DOIs
    Publication statusPublished - 6 Nov 2006

    Fingerprint

    Dive into the research topics of 'UV-induced fragmentation of Cajal bodies'. Together they form a unique fingerprint.

    Cite this